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Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans
An observation suggests that, during its shift from C-1 to C-2 of the nucleus, the side chain of the substrate remains bound to a site on the enzyme while a conformational change of the protein permits the necessary movement of the benzene ring. Expand
Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis
These studies demonstrate that organic compounds not previously reported to serve as growth substrates can be utilized by axenic cultures of microorganisms and possess novel degradative systems that are active toward other compounds whose biological degradation has been limited because of inherent structural considerations or because of low aqueous solubility. Expand
Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates
A no-headspace bottle assay was developed, allowing for direct and accurate determinations of aqueous TCE concentration, and consistent with Ks values determined for degradation, TCE significantly inhibited phenol degradation. Expand
Creosote-contaminated sites. Their potential for bioremediation
Bioremediation of creosote-contaminated materials is reviewed here by characterizing coal-tar creosote, identifying techniques for assessing the biodegradability of its many chemical constituents,Expand
Novel Pathway of Toluene Catabolism in the Trichloroethylene-Degrading Bacterium G4
Formation of a hybrid species of O- and O-labeled 3-methylcatechol from toluene in an atmosphere of O(2) and O( 2) established that G4 catabolizes toluenes by successive monooxygenations at the ortho and meta positions. Expand
Purification and properties of gentisate 1,2-dioxygenase from Moraxella osloensis
Gentisate 1,2-dioxygenase showed broad substrate specificity and attacked a range of halogen- and alkyl-substituted gentisic acids, but substituted maleylpyruvates were not attacked under these conditions. Expand
Metabolism of resorcinylic compounds by bacteria: alternative pathways for resorcinol catabolism in Pseudomonas putida
Data is presented which shows that one strain of Pseudomonas putida ORC catabolizes resorcinol by a metabolic pathway via hydroxyquinol and ortho oxygenative cleavage to give maleylacetate, but that the other strain (O1) yields mutants that utilize res orcinol. Expand
Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions
The reactions involved in the bacterial metabolism of naphthalene to salicylate have been reinvestigated by using recombinant bacteria carrying genes cloned from plasmid NAH7, and the gene order for the nah operon was shown to be p, A, B, F, C, E, D. Expand
Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli
A number of laboratory strains and clinical isolates of Escherichia coli utilized several aromatic acids as sole sources of carbon for growth and enzymatic reaction mixtures showed that all enzymes of this branched pathway were inducible. Expand
Oxidation of naphthenoaromatic and methyl-substituted aromatic compounds by naphthalene 1,2-dioxygenase
The action of naphthalene dioxygenase on a variety of methyl-substituted aromatic compounds resulted in the formation of benzylic alcohols, i.e., methyl group monooxygenation products, which were subsequently converted to the corresponding carboxylic acids by dehydrogenase(s) in the host strain. Expand