Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans
- W. Hareland, R. Crawford, P. Chapman, S. Dagley
- Biology, ChemistryJournal of Bacteriology
- 1 January 1975
An observation suggests that, during its shift from C-1 to C-2 of the nucleus, the side chain of the substrate remains bound to a site on the enzyme while a conformational change of the protein permits the necessary movement of the benzene ring.
Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis
- J. Mueller, P. Chapman, B. Blattmann, P. H. Pritchard
- Chemistry, BiologyApplied and Environmental Microbiology
- 1 April 1990
These studies demonstrate that organic compounds not previously reported to serve as growth substrates can be utilized by axenic cultures of microorganisms and possess novel degradative systems that are active toward other compounds whose biological degradation has been limited because of inherent structural considerations or because of low aqueous solubility.
Creosote-contaminated sites. Their potential for bioremediation
The approach is developed as a model system to project the potential application of bioremediation to ameliorate environments contaminated by complex mixtures of structurally diverse hazardous chemicals.
Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates
- B. Folsom, P. Chapman, P. H. Pritchard
- Biology, EngineeringApplied and Environmental Microbiology
- 1 May 1990
A no-headspace bottle assay was developed, allowing for direct and accurate determinations of aqueous TCE concentration, and consistent with Ks values determined for degradation, TCE significantly inhibited phenol degradation.
Novel Pathway of Toluene Catabolism in the Trichloroethylene-Degrading Bacterium G4
- M. Shields, S. Montgomery, P. Chapman, S. Cuskey, P. H. Pritchard
- Biology, ChemistryApplied and Environmental Microbiology
- 1 June 1989
Formation of a hybrid species of O- and O-labeled 3-methylcatechol from toluene in an atmosphere of O(2) and O( 2) established that G4 catabolizes toluenes by successive monooxygenations at the ortho and meta positions.
Purification and properties of gentisate 1,2-dioxygenase from Moraxella osloensis
Gentisate 1,2-dioxygenase showed broad substrate specificity and attacked a range of halogen- and alkyl-substituted gentisic acids, but substituted maleylpyruvates were not attacked under these conditions.
Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions
The reactions involved in the bacterial metabolism of naphthalene to salicylate have been reinvestigated by using recombinant bacteria carrying genes cloned from plasmid NAH7, and the gene order for the nah operon was shown to be p, A, B, F, C, E, D.
Metabolism of resorcinylic compounds by bacteria: alternative pathways for resorcinol catabolism in Pseudomonas putida
Data is presented which shows that one strain of Pseudomonas putida ORC catabolizes resorcinol by a metabolic pathway via hydroxyquinol and ortho oxygenative cleavage to give maleylacetate, but that the other strain (O1) yields mutants that utilize res orcinol.
Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli
A number of laboratory strains and clinical isolates of Escherichia coli utilized several aromatic acids as sole sources of carbon for growth and enzymatic reaction mixtures showed that all enzymes of this branched pathway were inducible.
A SURVEY OF THE STEROL COMPOSITION OF THE MARINE DINOFLAGELLATES KARENIA BREVIS, KARENIA MIKIMOTOI, AND KARLODINIUM MICRUM: DISTRIBUTION OF STEROLS WITHIN OTHER MEMBERS OF THE CLASS DINOPHYCEAE1
In this survey of the sterol signatures of more than 40 dinoflagellates, sterols with the properties of ED and NED were found in cultures of K. brevis and shown also to be the principal sterols of Karenia mikimotoi Hansen and Moestrup and Karlodinium micrum Larsen.