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Hydrogen bonding and biological specificity analysed by protein engineering
The role of complementary hydrogen bonding as a determinant of biological specificity has been examined by protein engineering of the tyrosyl-tRNA synthetase. Deletion of a side chain between enzymeExpand
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'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization.
'Knobs-into-holes' was originally proposed by Crick in 1952 as a model for the packing of amino acid side chains between adjacent alpha-helices. 'Knobs-into-holes' is demonstrated here as a novel andExpand
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Improved oligonucleotide site-directed mutagenesis using M13 vectors.
An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides. The DNA is first cloned into a novel M13 vector (based upon M13mp18 or M13mp19),Expand
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Stable heterodimers from remodeling the domain interface of a homodimer using a phage display library.
Structure-guided phage display was used to select for combinations of interface residues for antibody C(H)3 domains that promote the formation of stable heterodimers. A C(H)3 "knob" mutant was madeExpand
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The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)
In a previous study, a mutant of tyrosyl-tRNA synthetase in which a threonine residue (Thr51) was converted to proline dramatically improved the affinity of the enzyme for its ATP substrate. How doesExpand
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Substrate‐assisted catalysis: Molecular basis and biological significance
Substrate‐assisted catalysis (SAC) is the process by which a functional group in a substrate contributes to catalysis by an enzyme. SAC has been demonstrated for representatives of three major enzymeExpand
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Binding interactions of kistrin with platelet glycoprotein IIb‐IIIa: Analysis by site‐directed mutagenesis
The binding interactions between platelet fibrinogen receptor, glycoprotein (GP) IIb‐IIIa, and kistrin, a snake venom disintegrin protein that contains the adhesion site recognition sequenceExpand
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Crystal structure of Staphylococcus aureus tyrosyl‐tRNA synthetase in complex with a class of potent and specific inhibitors
SB‐219383 and its analogues are a class of potent and specific inhibitors of bacterial tyrosyl‐tRNA synthetases. Crystal structures of these inhibitors have been solved in complex with theExpand
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Contribution of domain interface residues to the stability of antibody CH3 domain homodimers.
Dimers of CH3 domains from human IgG1 were used to study the effect of mutations constructed at a domain-domain interface upon domain dissociation and unfolding, "complex stability". AlanineExpand
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Functional interaction among catalytic residues in subtilisin BPN′
Variant of the serine protease, subtilisin BPN′, in which the catalytic triad residues (Ser‐221, His‐64, and Asp‐32) are replaced singly or in combination by alanine retain activities with theExpand
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