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Purification and Characterization of Cystathionine (gamma)-Lyase from Lactococcus lactis subsp. cremoris SK11: Possible Role in Flavor Compound Formation during Cheese Maturation.
A cystathionine (gamma)-lyase (EC ((gamma)-CTL) was purified to homogeneity from a crude cell extract of Lactococcus lactis subsp. cremoris SK11 by a procedure including anion-exchangeExpand
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Medium-dependent regulation of proteinase gene expression in Lactococcus lactis: control of transcription initiation by specific dipeptides.
Transcriptional gene fusions with the Escherichia coli beta-glucuronidase gene (gusA) were used to study the medium- and growth-dependent expression of the divergently transcribed genes involved inExpand
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Inhaled, dual release liposomal ciprofloxacin in non-cystic fibrosis bronchiectasis (ORBIT-2): a randomised, double-blind, placebo-controlled trial
Background The delivery of antipseudomonal antibiotics by inhalation to Pseudomonas aeruginosa-infected subjects with non-cystic fibrosis (CF) bronchiectasis is a logical extension of treatmentExpand
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Diversity of cell envelope proteinase specificity among strains of Lactococcus lactis and its relationship to charge characteristics of the substrate-binding region.
The biochemical and genetical diversity of the subtilisin-like cell envelope proteinase (CEP) among Lactococcus lactis strains was investigated. The specificities of the proteinases of 16 strainsExpand
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Aminopeptidase Activities of Starter and Non-Starter Lactic Acid Bacteria under Simulated Cheddar Cheese Ripening Conditions
Abstract The effects of pH, NaCl and CaCl2 concentration on aminopeptidase activities of lactic acid bacteria were investigated in order to assess the potential role of these enzymes on proteolysisExpand
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Deletion of Various Carboxy-Terminal Domains ofLactococcus lactis SK11 Proteinase: Effects on Activity, Specificity, and Stability of the Truncated Enzyme
ABSTRACT The Lactococcus lactis SK11 cell envelope proteinase is an extracellular, multidomain protein of nearly 2,000 residues consisting of an N-terminal serine protease domain, followed by variousExpand
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Cloning and sequencing of the peroxisomal amine oxidase gene from Hansenula polymorpha.
We have cloned the AMO gene, encoding the microbody matrix enzyme amine oxidase (EC from the yeast Hansenula polymorpha. The gene was isolated by differential screening of a cDNA library,Expand
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A novel dairy product fermented with Propionibacterium freudenreichii improves the riboflavin status of deficient rats.
OBJECTIVE Riboflavin deficiency is common in many parts of the world, particularly in developing countries. The use of riboflavin-producing strains in the production of dairy products such asExpand
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Proteinase overproduction in Lactococcus lactis strains: regulation and effect on growth and acidification in milk.
Multicopy plasmids that contained the complete of 3'-deleted forms of the proteinase (prtP) gene of Lactococcus lactis subsp. cremoris SK11 under the control of different promoters were constructedExpand
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Starter culture development for improving the flavour of Proosdij-type cheese
The use of the additional mesophilic strain B851, which has specific flavour forming abilities, was tested for improving the flavour development of a Proosdij-type cheese made with a combination ofExpand
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