Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation.
This communication examines an extension to a two-dimensional distribution of sedimentation coefficient and frictional ratio, c(s,f(r), which is representative of a more general set of size-and-shape distributions, including mass-Stokes radius distributions, c (M,R(S)), and sedimentation coefficients-molar mass distributions c( s,M).
Studying multisite binary and ternary protein interactions by global analysis of isothermal titration calorimetry data in SEDPHAT: Application to adaptor protein complexes in cell signaling
A new computational platform for the global analysis of isothermal titration calorimetry (ITC) data for the study of binary and ternary multisite interactions, implemented as part of the public domain multimethod analysis software SEDPHAT is described.
On the distribution of protein refractive index increments.
Use of saturation mutagenesis to localize probable functional domains in the NahR protein, a LysR-type transcription activator.
Automated Dating of the World’s Language Families Based on Lexical Similarity
A computerized alternative to glottochronology for estimating elapsed time since parent languages diverged into daughter languages is described, and time depths for nearly all the world’s recognized language families and for many subfamilies are offered.
Co-repressor induced order and biotin repressor dimerization: a case for divergent followed by convergent evolution.
Dual strategies for peptidoglycan discrimination by peptidoglycan recognition proteins (PGRPs).
- C. P. Swaminathan, P. Brown, R. Mariuzza
- BiologyProceedings of the National Academy of Sciences…
- 17 January 2006
It is demonstrated that these innate immune receptors use dual strategies to distinguish between PGNs from different bacteria: one based on the composition of the PGN peptide stem and another that senses the peptide bridge crosslinking the stems.
Characterizing Protein‐Protein Interactions by Sedimentation Velocity Analytical Ultracentrifugation
This unit introduces the basic principles and practice of sedimentation velocity analytical ultracentrifugation for the study of reversible protein interactions, such as the characterization of…
A new adaptive grid-size algorithm for the simulation of sedimentation velocity profiles in analytical ultracentrifugation
On the analysis of sedimentation velocity in the study of protein complexes
Surprisingly, it is found that large errors can be caused by the finite scanning speed of the commercial absorbance optics, exceeding the statistical errors in the measured sedimentation coefficients by more than an order of magnitude.