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Structural and Functional Characterization of Disulfide Isoforms of the Human IgG2 Subclass*
In the accompanying report ( Wypych, J., Li, M., Guo, A., Zhang, Z., Martinez, T., Allen, M. J., Fodor, S., Kelner, D. N., Flynn, G. C., Liu, Y. D., Bondarenko, P. V., Ricci, M. S., Dillon, T. M.,Expand
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An improved trypsin digestion method minimizes digestion-induced modifications on proteins.
Trypsin digestion can induce artificial modifications such as asparagine deamidation and N-terminal glutamine cyclization on proteins due to the temperature and the alkaline pH buffers used duringExpand
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G/U and certain wobble position mismatches as possible main causes of amino acid misincorporations.
A mass spectrometry-based method was developed to measure amino acid substitutions directly in proteins down to a level of 0.001%. When applied to recombinant proteins expressed in Escherichia coli,Expand
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Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry.
The objective of this study was to determine if liquid chromatography mass spectrometry (LC/MS) data of tryptic digests of proteins can be used for quantitation. In theory, the peak area of peptidesExpand
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High-mannose glycans on the Fc region of therapeutic IgG antibodies increase serum clearance in humans.
Glycan structures attached to the C(H)2 domain of the Fc region of immunoglobulin G (IgG) are essential for specific effector functions but their role in modulating clearance is less clear. ClearanceExpand
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Identification and characterization of deamidation sites in the conserved regions of human immunoglobulin gamma antibodies.
Deamidation of asparagine residues of biological pharmaceuticals is a major cause of chemical degradation if the compounds are not formulated and stored appropriately. The mechanism of thisExpand
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Optimization of a reversed-phase high-performance liquid chromatography/mass spectrometry method for characterizing recombinant antibody heterogeneity and stability.
An enhanced analytical RP-HPLC/MS method was developed for monitoring the stability and production of intact and fragmented monoclonal antibodies (MAbs). The use of high column temperatures (70-80Expand
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Development of an analytical reversed-phase high-performance liquid chromatography-electrospray ionization mass spectrometry method for characterization of recombinant antibodies.
Analytical characterization of monoclonal antibodies has been hindered by the lack of appropriate chromatographic methods to be used in conjunction with high-resolution MS. Current methodologies forExpand
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Mass spectral study of polymorphism of the apolipoproteins of very low density lipoprotein.
New isoforms of apolipoprotein (apo)C-I and apoC-III have been detected in delipidated fractions from very low density lipoprotein (VLDL) using matrix-assisted laser desorption (MALDI) andExpand
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Human IgG2 Antibodies Display Disulfide-mediated Structural Isoforms*
In this work, we present studies of the covalent structure of human IgG2 molecules. Detailed analysis showed that recombinant human IgG2 monoclonal antibody could be partially resolved intoExpand
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