• Publications
  • Influence
Identification, Characterization, and Crystal Structure of the Omega Class Glutathione Transferases*
A new class of glutathione transferases has been discovered by analysis of the expressed sequence tag data base and sequence alignment, named Omega, which exhibit an unusual N-terminal extension that abuts the C terminus to form a novel structural unit. Expand
Characterization of the human Omega class glutathione transferase genes and associated polymorphisms.
An investigation of the genomic organization of human GSTO1 identified a second actively transcribed member of the Omega class (GSTO1), and expression of GSTO2 mRNA was seen in a range of tissues, including the liver, kidney, skeletal muscle and prostate. Expand
An efficient system for high‐level expression and easy purification of authentic recombinant proteins
The development of an efficient expression system, utilizing the ubiquitin fusion technique, which allows convenient high yield and easy purification of authentic protein and should be amenable to high‐throughput applications. Expand
Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes.
The structure shows an overall similarity to the mu and pi class enzymes particularly in the glutathione-binding domain, but the main difference concerns the extended C terminus of the alpha class enzyme which forms an extra alpha-helix that blocks one entrance to the active site and makes up part of the substrate binding site. Expand
Glutathione transferases, regulators of cellular metabolism and physiology.
Human cytosolic GSTs have emerged as significant regulators of pathways determining cell proliferation and survival and as regulators of ryanodine receptors that are essential for muscle function. Expand
Parkinson's disease, pesticides, and glutathione transferase polymorphisms
GSTP1-1, which is expressed in the blood-brain barrier, may influence response to neurotoxins and explain the susceptibility of some people to the parkinsonism-inducing effects of pesticides. Expand
Characterization of the omega class of glutathione transferases.
The Omega class of cytosolic glutathione transferases was initially recognized by bioinformatic analysis of human sequence databases, and orthologous sequences were subsequently discovered in mouse,Expand
A Role for Glutathione Transferase Omega 1 (GSTO1-1) in the Glutathionylation Cycle
It is found that GSTO1-1 is associated with the rapid glutathionylation of cellular proteins when the cells are exposed to S-nitrosoglutathione, and a common mechanism where it can act on multiple proteins and pathways is revealed. Expand
Nomenclature for mammalian soluble glutathione transferases.
The nomenclature for human soluble glutathione transferases (GSTs) is extended to include new members of the GST superfamily that have been discovered, sequenced, and shown to be expressed. The GSTExpand
Reversal of the glycolytic phenotype by dichloroacetate inhibits metastatic breast cancer cell growth in vitro and in vivo
It is demonstrated that DCA has anti-proliferative properties in addition to pro-apoptotic properties, and can be effective against highly metastatic disease in vivo, highlighting its potential for clinical use. Expand