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Reconstitution of a microtubule plus-end tracking system in vitro
TLDR
In vitro reconstitution of a minimal plus-end tracking system consisting of the three fission yeast proteins Mal3, Tip1 and the kinesin Tea2 is reported and it is shown that the EB1 homologue Mal3 has an enhanced affinity for growing microtubules end structures as opposed to the microtubule lattice, which allows it to track growingMicrotubule ends autonomously by an end recognition mechanism. Expand
CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites
TLDR
Re reconstitute a vertebrate plus end tracking system composed of the most prominent +TIPs, end-binding protein 1 (EB1) and CLIP-170, in vitro and dissect their end-tracking mechanism, finding that EB1 autonomously recognizes specific binding sites present at growing microtubule ends. Expand
A Minimal Midzone Protein Module Controls Formation and Length of Antiparallel Microtubule Overlaps
TLDR
This work mechanistically explain how the two conserved, essential midzone proteins PRC1 and Xklp1 cooperate to constitute a minimal protein module capable of dynamically organizing the core structure of the central anaphase spindle. Expand
GTPγS microtubules mimic the growing microtubule end structure recognized by end-binding proteins (EBs)
TLDR
It is demonstrated here that a closed microtubule B lattice with incorporated GTPγS, a slowly hydrolyzable GTP analog, can mimic the natural EB protein binding site and indicate that the guanine nucleotide γ-phosphate binding site is crucial for determining the affinity of EBs for lattice-incorporated tubulin. Expand
Interpersonal consequences of the pursuit of safety.
TLDR
The results supported a cognitive model of social anxiety, rather than alternative explanations, and elicited significantly more negative responses from others in the negative appraisal condition, where they employed safety behaviors than in the positive appraisals condition,Where they did not. Expand
Poleward transport of Eg5 by dynein–dynactin in Xenopus laevis egg extract spindles
TLDR
It is found that dynein-dynactin transports the essential motor Eg5 toward the spindle poles in Xenopus laevis egg extract spindles, revealing a direct interplay between two motors of opposite directionality. Expand
Phosphorylation relieves autoinhibition of the kinetochore motor Cenp-E.
TLDR
A model of dynamic control of Cenp-E motility, and hence chromosome congression, dependent upon phosphorylation at the kinetochore is suggested. Expand
Directional Switching of the Kinesin Cin8 Through Motor Coupling
TLDR
It is found that purified kinesin-5 Cin8 from budding yeast could behave as a bidirectional kinesIn, which can change directionality of movement depending on whether it acts alone or in an ensemble. Expand
Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor, and +TIP dynamics.
TLDR
Fluorescence microscopy-based in vitro assays on chemically functionalized glass slides for the study of several aspects of microtubule cytoskeleton dynamics are described, which can be performed in the presence of high concentrations of soluble proteins or even cell extract, which allows thestudy of complex protein mixtures that were previously not accessible to quantitative imaging in vitro. Expand
Obstacles on the microtubule reduce the processivity of Kinesin-1 in a minimal in vitro system and in cell extract.
TLDR
It is found that mGFP-labeled minimal dimeric kinesin-1 detaches prematurely from microtubules when it encounters obstacles, leading to a strong reduction of its processivity, a behavior that is largely independent of the type of obstacle used here. Expand
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