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Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
Circular (e.g. simian virus 40) and linear (e.g. λ phage) DNAs have been labeled to high specific radioactivities (>108 cts/min per μg) in vitro using deoxynucleoside [α-32P]triphosphates (100 to 250Expand
Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
A bacterial gene (neo) conferring resistance to neomycin-kanamycin antibiotics has been inserted into SV40 hybrid plasmid vectors and introduced into cultured mammalian cells by DNA transfusion.Expand
Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins.
The repair of DNA double-strand breaks in Saccharomyces cerevisiae requires genes of the RAD52 epistasis group, of which RAD55 and RAD57 are members. Here, we show that the x-ray sensitivity of rad55Expand
Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli.
We have developed methods for covalently joining duplex DNA molecules to one another and have used these techniques to construct circular dimers of SV40 DNA and to insert a DNA segment containingExpand
Electroporation for the efficient transfection of mammalian cells with DNA.
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field areExpand
Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.
  • R. Mulligan, P. Berg
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 April 1981
Cultured monkey (TC7) and mouse (3T6) cells synthesize an Excherichia coli enzyme, xanthine-guanine phosphoribosyltransferase (XGPRT; 5-phospho-alpha-D-ribose-1-diphosphate:xanthineExpand
High-efficiency cloning of full-length cDNA.
A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedureExpand
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
This paper describes a plasmid vector for cloning cDNAs in Escherichia coli; the same vector also promotes expression of the cDNA segment in mammalian cells. Simian virus 40 (SV40)-derived DNAExpand
Comparison of intron-dependent and intron-independent gene expression.
Recombinant simian virus 40 viruses carrying rabbit beta-globin cDNA failed to express the beta-globin sequence unless an intron was included in the transcription unit. The addition of eitherExpand
Studies of the Interaction between Rad52 Protein and the Yeast Single-Stranded DNA Binding Protein RPA
ABSTRACT The RFA1 gene encodes the large subunit of the yeast trimeric single-stranded DNA binding protein replication protein A (RPA), which is known to play a critical role in DNA replication.Expand