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Conservation of mechanism in three chorismate-utilizing enzymes.
A unifying mechanism for AS, ADCS, and IS entailing nucleophile addition to C2 of chorismate in an S(N)2' ' process is proposed. Expand
Lowering the entropic barrier for binding conformationally flexible inhibitors to enzymes.
The 420-fold increase in the binding affinity of PPi4 is attributed to a reduction in its conformational flexibility, thus providing the first rigorous measure of the entropic contribution to the binding energy in a protein-ligand complex and stressing the advantages of the design strategy. Expand
A General Method for Molecular Tagging of Encoded Combinatorial Chemistry Libraries
Acylcarbenes can be used to attach chemically inert, vanillin-linked tagging molecules directly to an unfunctionalized Merrifield polystyrene solid support and thus to allow encoding of combinatorialExpand
Virtual Hydrocarbon and Combinatorial Databases for Use with CAVEAT
Three new virtual databases have been developed for use with the bond-orientation-based database searching program CAVEAT. These consist of a database of trisubstituted monocyclic hydrocarbons havingExpand
Phosphonamidates as transition-state analogue inhibitors of thermolysin.
The correlation noted between inhibitor Ki and substrate Km/kcat is the most extensive one of this type, providing strong evidence that the phosphonamidates are transition- state analogues and not simply multisubstrate ground-state analogues. Expand
Purification and properties of cytidine deaminase from escherichia coli.
The enzyme is protected against inactivation in the presence of substrate, and the inhibition is reversed with high concentrations of mercaptoethanol, which suggests that inactivation is the result of a mercaptide formation between the mercury and an active-site thiol. Expand
Phosphorus amino acid analogues as inhibitors of leucine aminopeptidase.
The fact that tetrahedral phosphorus analogues are less potent inhibitors of LAP than they are of other zinc peptidases suggests that the mechanism of L AP may be fundamentally different than that of the latter enzymes. Expand
Crystal structure determination, refinement and molecular model of creatine amidinohydrolase from Pseudomonas putida.
The three-dimensional crystal structure of creatine amidinohydrolase (creatinase EC from Pseudomonas putida, a dimeric enzyme with a molecular weight of 97,000, has been determined byExpand