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New method for generating deletions and gene replacements in Escherichia coli.
We describe a method for generating gene replacements and deletions in Escherichia coli. The technique is simple and rapid and can be applied to most genes, even those that are essential. What makesExpand
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A novel sRNA component of the carbon storage regulatory system of Escherichia coli
Small untranslated RNAs (sRNAs) perform a variety of important functions in bacteria. The 245 nucleotide sRNA of Escherichia coli, CsrC, was discovered using a genetic screen for factors thatExpand
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Positive regulation of motility and flhDC expression by the RNA‐binding protein CsrA of Escherichia coli
Many species of bacteria devote considerable metabolic resources and genetic information to the ability to sense the environment and move towards or away from specific stimuli using flagella. InExpand
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CsrB sRNA family: sequestration of RNA-binding regulatory proteins.
Noncoding regulatory RNA molecules, also known as small RNAs, participate in several bacterial regulatory networks. The central component of the carbon storage regulator (Csr) and the homologousExpand
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Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E.
In Escherichia coli, the global regulatory protein CsrA (carbon store regulator A) binds to leader segments of target mRNAs, affecting their translation and stability. CsrA activity is regulated byExpand
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Post-transcriptional regulation on a global scale: form and function of Csr/Rsm systems.
Originally described as a repressor of gene expression in the stationary phase of growth, CsrA (RsmA) regulates primary and secondary metabolic pathways, biofilm formation, motility, virulenceExpand
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Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli.
The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilmExpand
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CsrA regulates glycogen biosynthesis by preventing translation of glgC in Escherichia coli
The carbon storage regulatory system of Escherichia coli controls the expression of genes involved in carbohydrate metabolism and cell motility. CsrA binding to glgCAP transcripts inhibits glycogenExpand
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CsrA post‐transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli
The RNA‐binding protein CsrA represses biofilm formation, while the non‐coding RNAs CsrB and CsrC activate this process by sequestering CsrA. We now provide evidence that the pgaABCD transcript,Expand
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Regulation of Bacterial Virulence by Csr (Rsm) Systems
SUMMARY Most bacterial pathogens have the remarkable ability to flourish in the external environment and in specialized host niches. This ability requires their metabolism, physiology, and virulenceExpand
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