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Structural Basis for Substrate Specificity of Escherichia coli Purine Nucleoside Phosphorylase*
Purine nucleoside phosphorylase catalyzes reversible phosphorolysis of purine nucleosides and 2′-deoxypurine nucleosides to the free base and ribose (or 2′-deoxyribose) 1-phosphate. Whereas the humanExpand
Tumor cell bystander killing in colonic carcinoma utilizing the Escherichia coli DeoD gene to generate toxic purines.
Inefficiency of gene delivery, together with inadequate bystander killing, represent two major hurdles in the development of a toxin-mediated gene therapy for human malignancy. The product of theExpand
The crystal structure of Streptococcus pyogenes uridine phosphorylase reveals a distinct subfamily of nucleoside phosphorylases.
Uridine phosphorylase (UP), a key enzyme in the pyrimidine salvage pathway, catalyzes the reversible phosphorolysis of uridine or 2'-deoxyuridine to uracil and ribose 1-phosphate or 2'-deoxyriboseExpand
Metabolism and metabolic actions of 6-methylpurine and 2-fluoroadenine in human cells.
Activation of purine nucleoside analogs by Escherichia coli purine nucleoside phosphorylase (PNP) is being evaluated as a suicide gene therapy strategy for the treatment of cancer. Because theExpand
6-Methylthioguanylic acid, a metabolite of 6-thioguanine.
Abstract Human epidermoid carcinoma (H. Ep. No. 2) cells, grown in culture in the presence of 6-thioguanine- 35 S or methionine[methyl- 14 C] plus unlabeled 6-thioguanine, contained a previouslyExpand
Metabolism and Metabolic Effects of Halopurine Nucleosides in Tumor Cells in Culture
Abstract Within a series of halo derivatives of adenosine, deoxyadenosine and arabinosyladenine attempts have been made to correlate structure with cytotoxicity, substrate activity for adenosineExpand
Novel DNA methyltransferase-1 (DNMT1) depleting anticancer nucleosides, 4′-thio-2′-deoxycytidine and 5-aza-4′-thio-2′-deoxycytidine
AbstractPurposeCurrently approved DNA hypomethylating nucleosides elicit their effects in part by depleting DNA methyltransferase I (DNMT1). However, their low response rates and adverse effectsExpand
Role of methylthioadenosine/S-adenosylhomocysteine nucleosidase in Vibrio cholerae cellular communication and biofilm development.
In Vibrio cholerae, the genes required for biofilm development are repressed by quorum sensing at high cell density due to the accumulation in the medium of two signaling molecules, choleraExpand
Formation and significance of 6-methylthiopurine ribonucleotide as a metabolite of 6-mercaptopurine.
Summary 6-Methylthiopurine (6-MeMP) ribonucleotide was identified as a metabolite of 6-mercaptopurine (6-MP) or of 6-MP ribonucleoside in cultured L1210 leukemia cells, adenocarcinoma 755 cells, andExpand
In vivo gene therapy of cancer with E. coli purine nucleoside phosphorylase.
We have developed a new strategy for the gene therapy of cancer based on the activation of purine nucleoside analogs by transduced E. coli purine nucleoside phosphorylase (PNP, E.C. 2.4.2.1). TheExpand
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