P. Thomas Iype

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Cultured rat liver cells induced a strong antibody response in syngeneic rats, directed against foetal calf serum components which were incorporated into the liver cell surface from the cell-culture medium. This antibody could be removed by absorption with liver cells or glutaraldehyde-fixed foetal calf serum. It is possible that the antigenic(More)
Using a multidisciplinary approach, we have measured various indicators of DNA damage in peripheral lymphocytes of human populations potentially at increased risk for cancer. Sister chromatid exchanges (SCE) and polycyclic aromatic hydrocarbon (PAH)-DNA adducts were evaluated in a group of firefighters; chromosomal aberrations and hprt mutations were(More)
A method for the in vitro identification of transformed rat liver epithelial and hepatoma cells was developed using the preferential microagglutination of concanavalin A (Con A) coupled to agarose beads (Con A:agarose) to their colonies. Con A:agarose attaches to the cell surface through a specific interaction of the Con A moiety and its receptors. The(More)
Although methapyrilene (MP) produces hepatocellular carcinomas in rats, it does not elicit many of the cellular responses induced by other hepatocarcinogens. We have investigated the early changes induced in rat liver epithelial cell cultures by MP using morphological, cytochemical, and cytofluorometric techniques. Within 2 h of MP treatment, inclusion(More)
The effect of the tumour promoters TPA, phenobarbitone and saccharin on the production of sister-chromatid exchanges (SCE) was studied. TPA produced a small but significant increase of SCE in Chinese hamster cell lines V-79 and CHO, and in a hybrid clone formed by fusion of CHO with rat liver epithelial cells. The enhancement of SCE by TPA was not affected(More)
European Communities, with the aim of evaluating screening tests in chemical car-cinogenesis. The first section contains papers dealing with general strategy and appraisal of current programmes for carcinogenesis screening in U.S.A. and Japan and the methods being used to evaluate short term tests. A word of warning is rightly sounded in this section by(More)
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