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We developed methodology to isolate and culture rat alveolar Type II cells under conditions that preserved their proliferative capacity, and applied lipofection to introduce an immortalizing gene into the cells. Briefly, the alveolar Type II cells were isolated from male F344 rats using airway perfusion with a pronase solution followed by incubation for 30(More)
The induction of sister chromatid exchanges (SCE) by the hepatocarcinogen methapyrilene hydrochloride was investigated using appropriate in vitro and in vivo mammalian cell systems. Methapyrilene, even at the maximum tolerated dose, did not induce SCE in Chinese hamster ovary cells (CHO) or when CHO cells or hamster lung fibroblasts, V-79, were cocultivated(More)
The effect of the tumour promoters TPA, phenobarbitone and saccharin on the production of sister-chromatid exchanges (SCE) was studied. TPA produced a small but significant increase of SCE in Chinese hamster cell lines V-79 and CHO, and in a hybrid clone formed by fusion of CHO with rat liver epithelial cells. The enhancement of SCE by TPA was not affected(More)
Monitoring human populations for specific DNA modifications has been made possible by developing highly sensitive immunoassays employing antibodies specific for carcinogen-DNA adducts. While these techniques have been used to follow occupationally and environmentally exposed populations, results have been limited by the lack of exposure data with which to(More)
The rate of uptake of 2-deoxyglucose in normal rat hepatic cells and chemically induced hepatoma cells in culture was studied. The hepatoma cells possessed a higher permeability to the sugar at all stages of culture. However, a decrease in the uptake of 2-deoxyglucose at confluency was observed in cells which exhibited density-dependent inhibition of(More)
A direct comparison has been made between normal parenchymal cells cultured from rat liver and malignant cells from both a rat liver tumor and a spontaneously transformed line derived from the parent rat culture. In all nondividing cells there was a 3- to 5-fold increase in the population of surface microvilli on the malignant cells compared to the normal(More)
Normal rat liver cell lines treated with the chemical carcinogen N-methyl-N-nitrosourea (MNU) elicited antibody production (detected by membrane immunofluorescence test in vitro) when injected into a highly inbred strain of rats from which the liver cells were originally isolated. In contrast, the control cells which were untreated did not evoke humoral(More)
Although methapyrilene (MP) produces hepatocellular carcinomas in rats, it does not elicit many of the cellular responses induced by other hepatocarcinogens. We have investigated the early changes induced in rat liver epithelial cell cultures by MP using morphological, cytochemical, and cytofluorometric techniques. Within 2 h of MP treatment, inclusion(More)