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An experimental approach is described that enables the analysis of interactions between exogenous surface ligands and components of the cytoplasm in neutrophil leukocytes. Neutrophils treated with the nonionic detergent Lubrol PX, under controlled conditions, yield intact detergent-insoluble ghosts. Morphological analysis of neutrophil ghosts shows that(More)
Experiments were performed to determine the effects of interrupting the flux of actin monomers between unpolymerised and polymerised pools in PtK2 cells by (1) microinjecting exogenous polymerisation nuclei and (2) blocking endogenous assembly sites with low concentrations of cytochalasin D. Fluorescent actin oligomers were prepared by glutaraldehyde(More)
  • P Sheterline
  • 1993
About half of the total actin in the cytoplasm of cultured animal cells is polymerised into filaments at any time. The filaments are further ordered into 3-dimensional patterns by their interaction with a number of actin-binding proteins (ABPs) to form the functional actin cytoskeleton. Three consistent patterns of organisation can be discerned both by(More)
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