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Presented here is an efficient algorithm for solving the bidomain equations describing myocardial tissue with active membrane kinetics. An analysis of the accuracy shows advantages of this numerical technique over other simple and therefore popular approaches. The modular structure of the algorithm provides the critical flexibility needed in simulation(More)
INTRODUCTION This simulation study presents a further inquiry into the mechanisms by which a strong electric shock fails to halt life-threatening cardiac arrhythmias. METHODS AND RESULTS The research uses a model of the defibrillation process that represents a sheet of myocardium as a bidomain. The tissue consists of nonuniformly curved fibers in which(More)
Insulin induces the enzyme tyrosine aminotransferase (TAT) in Reuber H-35 rat hepatoma cells. A clone of these cells (KRC-7) was used to study the relationship between changes in enzyme activity and hybridizable mRNA, and rates of transcription for TAT in response to insulin. Our results indicate that enzyme activity is inducible by insulin in the presence(More)
The goal of this simulation study is to examine, in a sheet of myocardium, the contribution of anode and cathode break phenomena in terminating a spiral wave reentry by the defibrillation shock. The tissue is represented as a homogeneous bidomain with unequal anisotropy ratios. Two case studies are presented in this article: tissue that can electroporate at(More)
The presence of bovine immunoglobulin in cell culture media, and its nature as a polyclonal antibody, imposes increasing difficulties in resolution from the MAb intended for in vivo human applications. Particular difficulties are encountered when murine MAbs are the target antibody. This study presents model cases to simulate this problem, and suggests an(More)
This article describes the purification and scale-up of ISIS 2302, a 20-mer phosphorothioate oligonucleotide by anion-exchange (AX) chromatography. The key operating parameters were optimized at gram scale and further scaled up to hundred gram. SOURCE 30Q, a high efficiency polymeric chromatographic media was used for both the small and large-scale work.(More)
To determine the degree of damage induced by different modes of agitation during storage of platelets for 5 days in polyolefin (PL-732), we studied pH, platelet count, release of lactic dehydrogenase (LDH) and beta-thromboglobulin (beta-TG), morphology and osmotic recovery. Platelets were maintained at 20-24 degrees C on elliptical, 6 rpm circular, 2 rpm(More)
Tyrosine aminotransferase (TAT) is a liver-specific enzyme whose activity is subject to positive regulation by several agents including insulin and agonists that increase the intracellular concentration of cAMP. To further characterize the mechanism of insulin action and the interaction between cAMP and insulin several types of experiments were performed in(More)
This modeling study demonstrates that a re-entrant activity in a sheet of myocardium can be extinguished by a defibrillation shock delivered via extracellular point-source electrodes which establish spatially non-uniform applied field. The tissue is represented as a homogeneous bidomain with unequal anisotropy ratios in the cardiac conductivities. Spiral(More)
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