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Mitochondrial distribution pattern after ultrarapid freezing in dimethylsulphoxide was assessed in human multipronucleate zygotes, 2-cell and 4-cell stage embryos using rhodamine 123. The mitochondrial distribution pattern was evaluated at 37 degrees C after a 30 min incubation in rhodamine 123 solution, 4 h and 24 h after thawing. Non-frozen human(More)
AIM OF THE STUDY Pilot study to analyse the efficacy and embryo morphology using a new human embryo culture medium (GM501) versus the conventional used medium (ISM1). METHODS Over a four-month period, all patients at the Leuven Institute of Fertility and Embryology (LIFE) were -randomly allocated to have their embryos cultured in either the standard(More)
Contrary to the belief that rapid cooling and thawing of mammalian embryos is detrimental to survival, it has been shown that under certain conditions mammalian embryos can survive rapid freezing-thawing. In this study, 237 human fertilized oocytes (93 pronucleates, 20 multipronucleates, and 124 cleaved embryos) were frozen for a period of 1 day to 7 months(More)
OBJECTIVE To evaluate in a prospective randomized study the outcome of subzonal insemination (SUZI) in patients with male subfertility. DESIGN In a period of 7 months, 48 patients underwent IVF treatment for male subfertility reasons. Normal insemination and SUZI were performed on sibling oocytes. Patients were divided into three groups depending on the(More)
OBJECTIVE To evaluate the results obtained after intracytoplasmic sperm injection (ICSI) in couples with male factor subfertility. DESIGN Retrospective analysis of results obtained after ICSI in the unit of in vitro fertilisation in a private centre for infertility. RESULTS Application of ICSI in treatment cycles for male subfertility resulted in a(More)
OBJECTIVE To assess embryo viability after ultrarapid freezing-thawing. DESIGN We studied the fluorescence pattern of 35 ultrarapidly frozen-thawed multipronucleate human embryos exposed to fluorescein diacetate. SETTING All the embryos were obtained from the Medical Center for Fertility Diagnostics and In Vitro Fertilization and Embryo Transfer at(More)
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