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The nucleotide sequence of 1.5 Mb of genomic DNA from Mycobacterium leprae was determined using computer-assisted multiplex sequencing technology. This brings the 2.8-Mb M. leprae genome sequence to approximately 66% completion. The sequences, derived from 43 recombinant cosmids, contain 1046 putative protein-coding genes, 44 repetitive regions, 3 tRNAs,(More)
DIRECT TRANSFER ELECTROPHORESIS 187 which usually takes less than an hour. The database should be monitored for random distribution of sequences; accumulation of specific sequences, which indicates nonrandom sonication or some cloning problem, will become apparent early on. In practice, however, sonication seems to produce random libraries from DNAs varying(More)
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