P. O. Michel

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KEY WORDS: CHROMOSOME / CHO CELLS / METAPHASE / KARYOTYPE / DIGITAL IMAGING BACKGROUND AND NOVELTY: Chinese hamster ovary (CHO)-derived cells are widely used for the production of recombinant proteins. Currently, the pharmaceutical industry relies on stable cell clones for therapeutic protein production. Most of the currently used methods to analyze(More)
Background Sf-9 cells, derived from Spodoptera frugiperda, are widely used for recombinant protein production using the baculovirus expression vector system (BEVS). However , this results in a productive viral infection and cell lysis. Therefore, a non-lytic, plasmid-based expression system for suspension Sf-9 cells would be a valuable alternative to the(More)
A major problem for the pharmaceutical industry is the failure of promising drug candidates very late in the testing phases. Often, there are unpredicted side-effects and toxicity issues which limit or prevent a candidate molecule being taken to market. Any testing regimes that can identify toxic molecules and exclude them from the screening programs as(More)
We designed two efficient and user-friendly tools for the seg-mentation and analysis of images containing chromosomes or, more generally, rod-shaped elements that are spread on microscopic slides. The segmentation tool allows to automatically extract the profile of each chromosome and to sort the collection of profiles in a karyotype image. The analysis(More)
We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (k L a) was 200%–400% higher in the HT tube than in a shake flask. Bacterial growth and plasmid production were 30% higher in the HT tube(More)
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