P. Nettesheim

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To identify genes upregulated during the process of ciliated cell differentiation of airway epithelial cells, differential display was used to compare RNA from rat tracheal epithelial (RTE) cells cultured under conditions that inhibit/promote ciliated cell differentiation. Several partial complementary DNAs (cDNAs) were identified whose expression was(More)
Retinoid-deficient cultures of airway epithelial cells undergo squamous differentiation. Treatment of such cultures with retinoic acid (RA) leads to restoration of the mucous phenotype. The purpose of our study was to characterize the cellular and molecular changes following RA treatment of retinoid-deficient human tracheobronchial epithelial cell cultures.(More)
The purpose of our studies was to examine differentiation-dependent expression of 15-lipoxygenase (15-LO) and prostaglandin H synthase (PGHS) isoforms in cultured normal human tracheobronchial epithelial cells. In the presence of retinoic acid (RA) the cultures differentiated into a mucociliary epithelium. When cultured in RA-depleted media, the cultures(More)
The aim of our studies was to analyze carcinogen dose effects on the development of "carcinogen-altered" preneo-plastic epithelial cell populations in rat tracheas following ex posure to 7,12-dimethylbenz(a)anthracene. As described pre viously (Cancer Res., 39: 4003-4010, 1979), cells appear in the trachéal epithelium upon exposure to carcinogen which are(More)
Several key events in the multistep process of neoplastic transformation of rat tracheal epithelium (RTE) are described. Whether tracheal epithelium is exposed in vivo to carcinogenic agents or whether primary tracheal epithelial cells are exposed in vitro to carcinogens, initiated stem cells can be detected soon after the exposure by their ability to grow(More)
Poverty and lack of development are critical contributing factors to environmental hazards that affect the health of many hundreds of millions of people worldwide, particularly at the household level. Too often in too much of the world, however, the industrial and commercial development that helps reduce poverty has resulted in ambient environmental(More)
MSC-10 tumour cells (derived from a chemically induced pulmonary squamous-cell carcinoma in DBA/2 mice) were inoculated intramuscularly into thymectomized, X-irradiated isogeneic mice, either 48 h or 6 weeks after thymectomy and X-irradiation. Normal mice and immunologically reconstituted mice served as controls. A marked enhancement in frequency of tumour(More)
The influence of vitamin A on the development of chemically induced lung carcinomas in rats was investigated. Rats were maintained on low, "normal" and excess levels of retinyl acetate (RA). Respiratory tract-squamous carcinomas were induced by intratracheal injections of 3-methylcholanthrene (3-MCA). The carcinogen doses used ranged from 1.25 to 10.0 mg of(More)
Rat tracheal epithelial (RTE) cells were cultured on membrane support with and without retinoic acid (RA). In early (6-day-old) cultures, the epithelium is a monolayer or bilayer of undifferentiated cells and secretes little mucuslike product either in the absence or presence of RA. In late (12- to 15-day-old) cultures, the epithelium differentiates as a(More)
Highly purified Clara cells (93 +/- 3%) isolated from the lungs of rabbits were used to produce an antiserum against Clara cell secretory proteins. This antiserum was used to identify and study the biosynthesis and secretion of [35S]methionine-labeled proteins from isolated Clara cells. The antiserum recognized one major secretory protein with apparent(More)