P. Martin Larsen

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Interleukin-1beta (IL-1beta) treatment of neonatal rat islets for 24 h induces changes in the expression of 105 of 2,200 proteins, as determined previously by two-dimensional (2D) gel electrophoresis. Nitric oxide (NO) has been implicated as one of the mediators of IL-1beta effects in insulin-containing cell lines and rat islets. The aims of this study were(More)
INTRODUCTION Coarctation of the aorta (CoA) is associated with morbidity despite treatment. Although mechanisms remain elusive, abnormal hemodynamics and vascular biomechanics are implicated. We present a novel approach that facilitates quantification of coarctation-induced mechanical alterations and their impact on vascular structure and function, without(More)
Analysis by means of two-dimensional gel electrophoresis (IEF) of [32P]orthophosphate-labeled proteins from mitotic and interphase transformed amnion cells (AMA) has shown that keratins IEF 31 (Mr = 50,000; Hela protein catalogue number), 36 (Mr = 48,500), 44 (Mr = 44,000), 46 (Mr = 43,500), as well as vimentin (IEF 26; Mr = 54,000) are phosphorylated above(More)
Actin is transformed sarcoma 180 cells is composed of the nonmuscle beta and gamma species and of a third, more acidic stable variant termed zeta. Two-dimensional peptide analysis shows that zeta is similar to beta actin, differing in the mobility of only one tryptic peptide. Several lines of evidence indicate that zeta is not a modified beta-actin species.(More)
Mouse polyclonal antibodies have been raised against two human proteins (IEF [isoelectric focusing] 31, Mr = 50,000; IEF 46, Mr = 43,500) that have previously been shown to be present in HeLa cytoskeletons enriched in intermediate-sized filaments. Immunoprecipitation studies show that both proteins share common antigenic determinants with each other and(More)
Neomycin has been reported to inhibit polyphospho-inositide cycling by preventing the hydrolysis of phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-trisphosphate and 1,2-diacylglycerol. Inositol 1,4,5-trisphosphate, through the mobilization of calcium , and 1,2-diacylglycerol, through the activation of protein kinase C, trigger many physiological(More)
Sera from 95 MS patients and 227 control persons were examined by IEF to determine the Gc genotype. There was no correlation between MS and the distribution of Gc genotype (0.70 less than P less than 0.80) nor Gc alleles (0.80 less than P less than 0.90) although such a correlation is generally accepted on the basis of a smaller material. Furthermore, Gc(More)
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