P. Martiat

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Chromatography is commonly used as a purification method for synthetic oligodeoxyribonucleotides (1). Anion exchange chromatography on Mono Q column (Pharmacia), under denaturing conditions and using non-organic buffers, gives adequate purity for sequences up to 50 bases long (2). The eluting NaCl concentration primarily depends on the oligonucleotide(More)
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