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Lead chloride, at concentrations of 67 muM in the incubation media, inhibits the potassium-stimulated respiration (3 to 30mM increase in K+) of rat cerebral cortex slices. The inhibition observed was dependent upon the substrate provided. In the presence of 10mM glucose or lactate, 35-50% inhibition of the response was observed, but no evidence of an effect… (More)
The isolation, extraction, and spectrophotometric determination of acetylcholine from Lactobacillus plantarum ATCC 10241 is described. Acetylcholine was extracted with a mixture of sodium tetraphenylboron-butylethylketone-acetonitrile and was measured enzymatically at 340 nm.