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A simple method for fast fractionation of human postmortem brain total poly(A)+RNA has been developed. Guanidine thiocyanate extractable RNA was directly applied on the membranes of cellulose nitrate with immobilized oligo(dT) chains. After the electrophoresis in 35 mM tris-acetete buffer (pH 7.90) containing 10 mM EDTA and 35% formamide, the membranes were… (More)
Rates of ATP production and protein synthesis rates were simultaneously studied using the isolated rat brain mitochondria and different types of brain polysomes (total, free, microsomal and mitochondrial membrane-bound). Protein synthesis was measured using a special reticulocyte lysate cell-free incubation medium. Prior to incubation, the intact… (More)