P. H. Cleat

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An enzyme-linked immunosorbent assay (ELISA) was developed to measure serum antibodies to group B streptococci in 20 healthy pregnant women before delivery and in their newborn infants. The sera from 10 of these women who were colonised with group B streptococci and umbilical cord sera from their infants, had higher levels of type-specific lgG antibody than(More)
Total-cell DNA isolated from a highly virulent serotype Ic strain of a group B streptococcus was used to construct a gene bank with bacteriophage lambda L47.1 in Escherichia coli K-12. Recombinant phage plaques in the bank were immunoblotted by using anti-alpha- and anti-beta-specific antibodies directed towards the Ibc proteins purified from the(More)
The phagocytic and bactericidal activities of normal adult human neutrophils against 2 strains of Group B streptococci Type Ic of differing pathogenicity were examined. Both isolates were phagocytosed by the neutrophils in the presence of normal and homologous immune serum. However, the highly pathogenicity streptococci were killed less readily in the(More)
IgG fractions were separated by ion exchange chromatography on DEAE-cellulose from hyperimmune rabbit sera prepared against a group Bstreptococcus type Ia mouse-virulent strain. 50Μg IgG in conjunction with ampicillin (200 mg/kg) protected mice more effectively against a lethal challenge than ampicillin (400 mg/kg) alone or ampicillin (200 mg/kg) combined(More)
An investigation was undertaken into the role of antibody and complement in neutrophil phagocytosis and killing of group B streptococci of low and high pathogenicity. Phagocytosis of both types of organism appears to be a nonspecific event requiring only nonspecific antibody or complement. However, neutrophil bactericidal activity is mediated by the(More)
The DNA-protein and protein-protein interactions proposed for the stability of nucleoprotein complexes at the origin of replication in prokaryotes are also thought to impart regulatory precision in eukaryotic DNA replication. This type of specificity can be observed, for example, during adenovirus DNA replication where efficient initiation requires that(More)
An electron microscopic study was undertaken of the entry of specific antibody into neutrophils containing surviving intracellular highly virulent group B streptococci after phagocytosis of the organisms had occurred. Electron micrographs are presented to demonstrate that specific antibody gains access to the ingested bacteria. This antibody binds to the(More)
An investigation was undertaken into the post-phagocytic action of specific antibody in initiating neutrophil bactericidal activity against highly pathogenic group-B streptococci that survive within these cells. Specific antibody appears to gain access to the interior of the neutrophil and binds to the intracellular bacteria. Activation of Fc receptors on(More)
Group B streptococcus can be a serious infection of neonates and, in the past, specific antibody has been shown to mediate immunity against this organism. However, specific antibody is not required for phagocytosis of highly pathogenic organisms by neutrophils. Furthermore, the bacteria subsequently survive and tend to accumulate or multiply in these cells,(More)
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