P. Gurusaravanan

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An efficient and reproducible two-step in vitro propagation system for tomato (Lycopersicon esculentum) was developed by using the combinations of seaweed biostimulant (Gracilaria edulis and Sargassum wightii) extracts and plant growth regulators (PGRs). Double cotyledonary nodal (DCN) explants of Co-3 cultivar were initially cultured on Murashige and Skoog(More)
In the present study a simple and efficient somatic embryogenesis system was developed from leaf explants of Lycopersicon esculentum L. The protocol has been developed by using plant growth regulators and seaweed extracts a natural biostimulant. The leaf sections were initially cultured on to leaf embryogenic callus induction medium fortified with various(More)
Unfortunately, Figures 1 and 2 were interchanged when this article was produced and should appear a shown here. Fig. 1 Effect of MS basal medium, Gracilaria edulis and Sargassum wightii on in vitro seed germination of tomato. The medium was composed of MS salts and B5 vitamins, 3% sucrose and seaweeds at different concentrations. MS medium without any(More)
A simple and efficient protocol for Agrobacterium-mediated genetic transformation of tomato was developed using combination of non-tissue culture and micropropagation systems. Initially, ESAM region of 1-day-old germinated tomato seeds were microinjected for one to five times with Agrobacterium inoculums (OD(600) = 0.2-1.0). The germinated seeds were(More)
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