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Fine structural cytochemistry and immunocytochemistry were used to study nucleic acids and nuclear proteins in nuclear bodies (NB) of pronuclear and 2-cell bovine and caprine embryos on ultrathin sections of paraformaldehyde fixed and Lowicryl K4M or LR White embedded specimens. The most striking feature detected in some of these nuclear bodies (NBs) was(More)
The fine structure of pig oocytes at the germinal vesicle (GV) stage and early preimplantation embryos (one to four blastomeres) isolated at slaughter was investigated by cytochemical and immunocytochemical methods. The distribution of nucleic acids and ribonucleoproteins (RNPs) in “compact nucleoli” [denominated nucleolus-like bodies (NLB) in oocytes and(More)
Early bovine precompacted embryos (at 1- to 8-blastomere stage) were analyzed by electron microscopy. The volume density of cellular components was determined by morphometric analysis to quantify the ultrastructure of early bovine embryos produced either in vivo or parthenogenetically after stimulation of oocytes by electric pulse AC/DC. In embryos obtained(More)
The aim of the present study was to evaluate the development and ultrastructure of preimplantation bovine embryos that were exposed to bovine viral diarrhea virus (BVDV) in vitro. The embryos were recovered from superovulated and fertilized Holstein-Friesian donor cows on day 6 of the estrous cycle. Compact morulae were microinjected with 20 pl of BVDV(More)
Heifers (n=31) were superovulated with an FSH-P/cloprostenol regimen, and at 12 and 24 hours after the onset of estrus they were inseminated. Blood sampling for LH analyses and ultrasound scanning of the ovaries were performed at 4-hours intervals. The scanning, at which the first and last ovulations were recorded, was performed at 22.7 +/- 1.5 (mean +/-(More)
Small nuclear ribonucleoproteins (snRNPs) were localized using human autoimmune or monoclonal anti-snRNP antibodies and ultrastructural immunocytochemistry in early preimplantation bovine embryos before and at transcription onset. Bovine cleavage stages up to 16-cell embryos were obtained either by culture of in vitro-fertilized ovarian oocytes or by(More)
The superovulation of donors, heifers and cows, was induced by means of gonadotropin PMSG (Antex Leo) in combination with the synthetic prostaglandin analogue--PGF2 alpha (Estrumate, ICI) applied 48 hours later. The response of donors to this treatment was variable. The average number of ovulating follicles from one heifer was fourteen, and the number of(More)
Local application of /5-3H/uridine or /6-3H/thymidine to the cow ovary was obtained by the instillation of the radioactive solution into a rubber balloon ('bursa') tightly enclosing the organ exposed by laparotomy. After 30-90 min the exteriorized ovary was replaced in the abdominal cavity. Ovaries were recovered 1-8 days later and processed for(More)
Approximately in 80% of cow oocytes (n = 632) ended cytoplasmatic and nucleus maturation to the state of metaphase II in the conditions of 24 hours in vitro cultivation. In 300 oocytes cytochemically we have determined the activity of enzymes--the succinate dehydrogenase (SDH, EC, alpha-glycerophosphate dehydrogenase (GPDH, EC and(More)