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1. Experiments were carried out to investigate the accumulation from the extracellular medium of 45Ca2+ by the endplate region of skeletal muscle. 2. Mouse diaphragm muscle was incubated in physiological saline labelled with 45Ca at 37 degrees C for periods of up to 1.5 h. 3. The muscle was divided into junctional and non-junctional portions and the Ca from… (More)
1. Experiments have been made on the phrenic nerve and diaphragm of rats and mice in vitro at 37 degrees C. 2. The administration of the anticholinesterase drugs ecothiopate, ambenonium or neostigmine resulted in enhancement of the 'twitch' response to nerve stimulation which was often followed by a prolonged contraction lasting several seconds. 3. The… (More)