P E Montandon

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A cDNA and a genomic DNA library from soybean (Glycine max L.) were used to identify and sequence two genes coding for the α-subunit of the translation elongation factor eEF-1. Within the coding part, the two genes (tefS1 andtefS2) diverge in 80 wobble positions thus yielding an identical protein composed of 447 amino acids. The soybean protein has about(More)
We retrieved a 1.6 kbp intron separating two exons of the psb C gene which codes for the 44 kDa reaction center protein of photosystem II. This intron is 3 to 4 times the size of all previously sequenced Euglena gracilis chloroplast introns. It contains an open reading frame of 458 codons potentially coding for a basic protein of 54 kDa of yet unknown(More)
The chloroplast genome of Euglena gracilis contains a supplementary gene for a 16 S rRNA (s16 S rrn gene), which is not part of a complete rrn operon. An open reading frame (ORF406) is located downstream of the s16 S rrn gene. Chloroplast RNA was hybridized with cloned DNA fragments of this region and the hybrids were analysed by electron microscopy and(More)
This replication method, which was introduced in 1985, has been used to find and identify microorganisms in the environment, among others in samples of soil, sediments and waters. A gene or a DNA fragment specific to a microorganism is replicated in vitro by a chain reaction catalyzed by DNA polymerase (PCR: Polymerase Chain Reaction) and analyzed by(More)
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