P. David Foglesong

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Purified human DNA topoisomerase I was assayed quantitatively by enzyme titrations with supercoiled pHC624 DNA in the presence of 0–2.0 μm doxorubicin. Supercoiled and relaxed DNAs were resolved by agarose gel electrophoresis in the presence of ethidium bromide, and the pereentage of conversion of supercoiled DNA to relaxed DNA was quantified by scanning(More)
Vaccinia virus cores contain a type I topoisomerase which promotes the relaxation of superhelical DNA of either handedness (Bauer et al., Proc. Natl. Acad. Sci. U.S.A. 74:1841-1845, 1977). The activity of partially purified vaccinia virus topoisomerase (VV-Topo I) was determined in the presence of ATP, dATP, GTP, ADP, and ATP analogs in which hydrolysis of(More)
Supercoiled and relaxed DNA were resolved electrophoretically in the presence of 0.5 micrograms/ml ethidium bromide. Under these conditions the Gaussian distributions of topological isomers of both supercoiled and relaxed DNA migrated as discrete bands. The separation of these DNAs was optimized by varying the concentration of electrode buffer.(More)
Anti-melanoma monoclonal antibody XMMME-001 was cross-linked to anti-CD3 monoclonal antibody OKT3 with succinimidyl 3(2-pyridyldithio)propionate (SPDP), and 2-iminothiolane. The dimer heteroconjugate was purified by HPLC gel filtration, labeled with131I, and 10 µg was injected into each of 24 BALB/c mice. The dimeric heteroconjugate's blood survival in(More)
Earlier studies have suggested that higher cellular levels of teniposide (VM-26) are required for the inhibition of growth in L1210/VM-26 sublines than in parental L1210 cells [8]. On the basis of this observation, we hypothesized that resistance to VM-26, which is partly attributed to multidrug resistance, also resulted in reduced formation of DNA lesions(More)
Multiple DNA-dependent enzyme activities have been detected in highly purified preparations of a single-strand-specific nuclease from vaccinia virus. These enzyme preparations were extensively purified and characterized by using superhelical DNAs as substrates. In particular, the nuclease activity was monitored by the extent of conversion of supercoiled(More)
DNA topoisomerase activity can be rapidly assayed by measuring the change in ethidium bromide fluorescence intensity after treatment of closed duplex DNA with enzyme. The sensitivity of the fluorometric assay has been enhanced 3-fold by a 10-fold reduction in ethidium bromide concentration to 0.1 microgram/ml. The results of the fluorometric assays are in(More)
A preliminary map of the frog virus 3 (FV 3) genome was constructed by hybridization-selection of mRNAs to cloned DNA fragments and translation in reticulocyte lysates. FV 3 mRNAs were hybridized toKpnI,HindIII, andSalI restriction fragments representing the entire FV 3 genome. Two different hybridization conditions were employed in order to discriminate(More)
We isolated a new larval color mutant, brown larva (b), from the Bangalore, India strain of Anopheles stephensi Liston. The gene b is an autosomal recessive with uniform expression and complete penetrance. We conducted extensive crosses to establish allelism between brown larva (b) and green larva (g) reported previously in An. stephensi from our(More)