P. C. Onvlee

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The use of Percoll-purified treponemes in an assay similar to the Treponema pallidum Immobilisation test demonstrated that immobilisation of purified treponemes by seronegative normal human serum proceeded at a much higher rate than that of unpurified treponemes. This suggests that the removal of the testicular extract makes the treponemes more vulnerable(More)
Since the introduction of the treponemal immobilization (TPI) test by NELSON and MAYER in 1949 a great number of communications concerning the value of this test for the diagnosis of syphilis have been published (MAGNUSON and THOMPSON, 1949; MOORE and MOHR, 1952; MILLER el al., 1954; DUREL el al., 1952; REYN and NIELSEN, 1952; CHACKO, 1953; WILKINSON, 1954(More)
An ultracentrifugally homogeneous heat-stable polysaccharide preparation free from serologically reactive rabbit testicular tissue antigen, including cardiolipin, was extracted from the Nichols strain of Treponema pallidum, and found to react by complement-fixation with homologous rabbit sera but not with human syphilitic sera. In addition, the reactive(More)
OBJECTIVES To study the role of different components in normal human serum and the role of lysozyme in rapid immobilisation of Percoll purified T pallidum (Nichols). MATERIALS AND METHODS The immobilisation of Percoll purified T pallidum was studied after pre-incubations with different serum fractions (Fr) of normal human serum (Fr 1, containing IgM; Fr(More)
The utility of the addition of an equal amount of a 10 % Na3PO4 solution to gastric lavages in culturing forMycobacterium tuberculosis has been investigated and has been found applicable under certain conditions, on the understanding that before and during transport the mixture is kept at temperatures not exceeding 20° C.
An ultracentrifugally homogeneous heat-stable polysaccharide preparation free from serologically reactive rabbit testicular tissue antigen, including cardiolipin, was extracted from the Nichols strain of Treponema pallidum, and found to react by complement-fixation with homologous rabbit sera but not with human syphilitic sera. In addition, the reactive(More)
OBJECTIVES Investigation of sera, especially rabbit serum, in preventing in vitro immobilisation of Percoll purified T. pallidum. MATERIALS AND METHODS The immobilisation of Percoll purified T. pallidum (Nichols) was studied after pre-incubations with basal reduced medium (BRM), heat-inactivated serum of seven different species of animals,(More)
Fresh and aged adherent T pallidum were used to study the accessibility of their outer membrane to antibodies by means of an indirect immunofluorescent technique. The integrity of the outer membrane was demonstrated by the non-reactivity with a monoclonal antibody directed against the axial filaments. Using the sera from patients with sero-positive primary(More)