Learn More
We evaluated three different quantitative evaluation methods for lymphocyte locomotion in three-dimensional collagen gels: (1) the length of the two-dimensional migration path (distance migrated) was compared to (2) the resulting average displacement from the starting to the end point and (3) the displacement of the furthest migrating population (cells with(More)
The invasion and migration occurring in primary neoplastic tissue explants were studied by using a three-dimensional collagen matrix model, subsequent time-lapse videomicroscopy, and computer-assisted cell tracking. We show that not only single cells but groups of clustered cells comprising 5 to more than 100 cells detach from the primary tumor lesion and(More)
The locomotory trajectories of MOS-11 cells migrating in a three-dimensional hydrated collagen lattice have been determined using a computer-assisted optical sectioning unit. The trajectories have been quantified using a three-dimensional continuous-time Markov probability theory consisting of eight directional states and one stationary state; in the latter(More)
T cell locomotion within the extracellular matrix may be mediated by cell adhesion molecules. We investigated the expression and function of beta 1- and beta 2-integrins and CD44 on human peripheral CD4+ and CD8+ lymphocytes locomoting in a 3-D type I collagen matrix. Paths of randomly selected T cells were digitized from time-lapse videorecordings and were(More)
Using a time-lapse cinephotomicrographic technique, the locomotory paths taken by lymphocytes have been shown to satisfy the requirements for a continuous-time Markov chain analysis consisting of four directional states defined by the four quadrants of a Cartesian plane and state 0 in which the cells are stationary. The important parameters characterizing(More)
It has been postulated that low energy (soft) lasers can enhance wound healing. Considering the importance of cell locomotion in the wound healing process, we have studied the effects of a helium/neon laser upon the locomotory behaviour of a population of fibroblasts migrating within a three-dimensional hydrated collagen lattice. Statistical methods were(More)
Three-dimensional locomotory trajectories have been determined for an embryonic fibroblast population and also for these cells after a 20-day period in tissue culture (C-20 cells). Differences in locomotory characteristics between these two cell populations are reported. Applying factor analysis to the distribution of angle changes between vectors in a(More)
A method is presented for analysing the vectors of cells locomoting within three-dimensional collagen gels. The method detected differences in locomotory vector patterns between cells locomoting in two different gel formats. The potential of this analytical method for determining the role of the extracellular matrix in modulating cell locomotory behaviour(More)