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Knowledge of the error structure of a given set of experimental data is a necessary prerequisite for incisive analysis and for discrimination between alternative mathematical models of the data set. A reaction system consisting of glutathione S-transferase A (glutathione S-aryltransferase), glutathione, and 3,4-dichloro-1-nitrobenzene was investigated under(More)
In vivo administration of pertussis toxin is often used to study the involvement of guanine nucleotide binding proteins in signal transduction. Especially when it is administered in the brain the effect is often poor. This could be due to the fact that pertussis toxin does not reach the area of interest. To evaluate the extent to which pertussis toxin is(More)
The development and duration of neutralizing antibodies (antitoxin) to pertussis toxin were studied in 38 patients with culture-verified infections due to Bordetella pertussis and one patient with infection due to Bordetella parapertussis. An in vitro neutralization test in microplate culture of Chinese hamster ovary cells was used. An antitoxin response(More)
Serum responses to oral cholera vaccines were assessed in three paediatric vaccine trials, two in León, Nicaragua and one in Stockholm, Sweden. A calibrated anti-cholera toxin B subunit (CTB) IgA ELISA was used together with an assay for vibriocidal antibodies. Swedish children had lower pre-vaccination levels of antibody, but serum responses were more(More)
Antibody responses in human sera against Bordetella pertussis during natural infection were investigated by a microplate enzyme-linked immunosorbent assay (ELISA) with a purified fimbrial haemagglutinin preparation as antigen. Significant rises of specific IgG, IgM and IgA were demonstrated in paired sera. A secondary type of antibody response was found in(More)
Induction of immunity against antigens expressed on tumor cells might prevent or delay recurrence of the disease. Six patients operated on for colorectal carcinoma were immunized with human monoclonal anti-idiotypic antibodies (h-Ab2) against the mouse 17-1A anti-colon carcinoma antibody, mimicking a nominal antigen (GA733-2). All patients developed a(More)
Diagnosis of whooping cough by an enzyme-linked immunosorbent assay (ELISA) that measures serum IgG, IgM, and IgA antibody to the fimbrial hemagglutinin of Bordetella pertussis was compared to isolation from nasopharyngeal swabs in a prospective study. Of 77 patients with upper respiratory tract infections of unknown etiology in which B. pertussis infection(More)