P. A. Levashov

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The bacteriolytic activity of interleukin-2 and hen egg white lysozyme against 34 different species of microorganisms has been studied. It was found that 6 species of microorganisms are lysed in the presence of interleukin-2. All interleukin-2-sensitive microorganisms belong either to the Enterobacteriaceae, Bacillaceae, or the Lactobacillaceae family. It(More)
In this paper we report the discovery of bacteriolytic activity of an immune system cytokine mediator, interleukin-2. Bacteriolytic activity of interleukin-2 was compared with a well-known bacteriolytic enzyme — chicken egg white lysozyme — by monitoring the lysis of the Gram-negative bacterium Escherichia coli, the Gram-positive coccus Micrococcus luteus,(More)
DNA aptamer-based sorbents were synthesized for binding human IgE. Sorbents effectively removed IgE from human blood plasma. The experimental values of IgE desorption constants were within the range of 1.1 × 10−10 to 1.7 × 10−10 M depending on the orientation of the aptamer on an insoluble matrix. The sorbents were stable during multiple use. Conditions for(More)
Hemocompatible affinity sorbents based on the polysaccharide matrix and WY, WTY, and WNY ligands for the binding of human immunoglobulins G (IgG) have been synthesized. The characteristics of the sorbents such as the binding of total IgG and IgG subclasses have been compared. It has been found that the novel sorbents easily extract IgG from the blood(More)
Bacteriolytic factors from the blood plasma of healthy sheep have been studied. Three enzymes not described earlier in the literature have been identified and characterized. Two enzymes exhibit activity toward Escherichia coli and have molecular weights of 15 ± 2 kDa. The third enzyme that exhibits activity toward E. coli and Micrococcus luteus has a(More)
A new procedure for the preparation of affinity sorbents based on tyramine and tryptamine ligands has been proposed. Sorption characteristics for the developed chromatographic materials were investigated. It was shown that tryptamine-sepharose and tyramine-sepharose efficiently bind IgG, IgA, lipoprotein (a) (Lp(a)) and low density lipoproteins (LDL) from(More)
The effect of surfactants of different types: anionic (sodium dodecyl sulfate, SDS), cationic (dodecyltrimethylammonium bromide, DTAB), and zwitterionic (cocoamidopropyl betaine, CAPB) on the lysis of Gram-positive bacteria Micrococcus luteus by chicken egg lysozyme at pH 7.2 and an ionic strength of the solution of 0.15 M has been investigated. It has been(More)
Chromatographic material based on tryptophil-threonyl-tyrosine and efficiently binding human, sheep, goat, and bovine immunoglobulin G was obtained. High selectivity of the sorbent for extraction of immunoglobulins from blood plasma has been demonstrated. Effective sorption capacity is 15–25 mg of immunoglobulin G per 1 ml of the matrix. Optimization of the(More)
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