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Formins are highly conserved proteins that are essential in the formation and regulation of the actin cytoskeleton. The formin homology 2 (FH2) domain is responsible for actin binding and acts as an important nucleating factor in eukaryotic cells. In this work EPR and DSC were used to investigate the properties of the mDia1-FH2 formin fragment and its(More)
Developments in nanotechnology and in the formulation of liposomal systems provide the opportunity for cosmetic dermatology to design novel delivery systems. Determination of their physico-chemical parameters has importance when developing a nano-delivery system. The present study highlights some technological aspects/characteristics of liposomes formulated(More)
The antibacterial efficacy of the broad spectrum fluoroquinolone antibacterial drug lomefloxacin (LMFX) has been investigated in a wide variety of clinical infections. An alternative approach to the classical delivery of antibacterial drugs consists in associating the drug to a liposomal carrier. It seems clear that liposomal encapsulated antibiotics allow(More)
The effects of clary sage (Salvia sclarea L.) oil (CS-oil), and its two main components, linalool (Lol) and linalyl acetate (LA), on cells of the eukaryotic human pathogen yeast Candida albicans were studied. Dynamic and thermodynamic properties of the plasma membrane were investigated by electron paramagnetic resonance (EPR) spectroscopy, with(More)
Optical waveguide lightmode spectroscopic (OWLS) techniques were probed for monitoring ion permeation through channels incorporated into artificial lipid environment. A novel sensor set-up was developed by depositing liposomes or cell-derived membrane fragments onto hydrophilic polytetrafluoroethylene (PTFE) membrane. The fibrous material of PTFE membrane(More)
Molecular dynamics of membrane proteins in frog nerve was studied by the spin labelling technique. By varying the reaction conditions three protein domains were identified which differ in polarity of the micro-environment of the labelled sites (polarity index, h = 0, 0.6 and 1.2) and in mobility (apparent rotational correlation times, tau 2 = 1 ns, 35 ns(More)
The decorated actin technique combined with the spin label method was used to study the interaction of skeletal muscle actin and myosin in synthetic filament system. If follows from the orientation dependence of the EPR spectra of the rigidly attached spin label in F-action filaments that the spin-label is sensitive to the dynamic state of the filaments.(More)
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