Ortansa Csutak

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Two rounds of chromosome segregation after only a single round of DNA replication enable the production of haploid gametes from diploid precursors during meiosis. To identify genes involved in meiotic chromosome segregation, we developed an efficient strategy to knock out genes in the fission yeast on a large scale. We used this technique to delete 180(More)
Synaptonemal complex (SC) proteins Hop1 and Mek1 have been proposed to promote homologous recombination in meiosis of Saccharomyces cerevisiae by establishment of a barrier against sister chromatid recombination. Therefore, it is interesting to know whether the homologous proteins play a similar role in Schizosaccharomyces pombe. Unequal sister chromatid(More)
Three Metschnikowia pulcherrima strains isolated from white grapes (strains designated SG1 and SG2) and cherries (strain CPM1) from Romania were tested for antagonistic activity against natural and reference yeasts, fungi and E. coli. The best results were obtained for SG2 strain against the following microbes isolated from natural environments: yeasts(More)
Besides their important biotechnological and industrial applications, yeasts have been used during the last years, in obtaining probiotic products, along with lactic acid bacteria and various enzymes. Our study deals with some aspects regarding the use of yeasts as animal and human probiotics, and their possible mechanisms of action. Also, we present(More)
Yeast identification has a real importance for selection of technological strains and also for studying biodiversity and population dynamics in the fermentation systems. Important studies on yeast population dynamics showed that Saccharomyces genus is dominant in alcoholic fermentation, while other genera like: Kloeckera, Candida, Pichia, Hansenula,(More)
Xylitol is a naturally occuring sugar alcohol, which is currently produced chemically on a large scale. In recent years the bioconversion of D-xylose from lignocellulosic residues into xylitol, gained an increased attention as an alternative procedure , due to its high efficiency and reduced cost of such technology. The biotechnological method of producing(More)
Due to the major impact of yeasts in human life based on the existence of pathogen yeast species and of species with biotechnological abilities, in the last few years new molecular techniques are performed for an accurate identification of natural isolates. Our study is aimed to review some of these techniques such as electrokariotyping by PFGE (Pulsed(More)
Bioremediation is a very interesting alternative for restoring the oil-polluted ecosystems. Many studies concerning the possibility of using microorganisms (bacteria and yeasts) in the degradation of oil compounds have as starting point the isolation and taxonomical identification of new species and strains with degradative abilities. Our study focusses on(More)
Yeast lipases present a growing interest for the synthesis of novel compounds and development of technologies with biotechnological applications. A new yeast strain CMGB-CR6 isolated from oilpolluted soil was identified as belonging to Candida rugosa using morphological observations, the API 20C AUX and Biolog Microbial ID systems and PCR-RFLP of the 5.8S(More)