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The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for heterologous protein production. The use of different phenotypes under PAOX promoter, other(More)
A Pichia pastoris strain expressing a Rhizopus oryzae lipase gene under the transcriptional control of the promoter from the P. pastoris formaldehyde dehydrogenase 1 gene (PFLD) was utilized to study(More)
An important number of heterologous proteins have been produced in the methylotrophic yeast Pichia pastoris using the alcohol oxidase promoter. Two factors that drastically influence protein(More)
Abstract A Rhizopus oryzae lipase (ROL) has been expressed in Pichia pastoris as a reporter gene using two different regulated promoters. Both phenotypes, Mut s and Mut + , have been used for the(More)