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Evaluation of loop-mediated isothermal amplification method for detecting enterotoxin A gene of Staphylococcus aureus in pork.
The LAMP assay allows a one-step identification of gene of interest without any specialized equipment and requires less time than conventional PCR, thus suggesting that the LAMP technique might be an appropriate alternative diagnostic method for detecting enterotoxin A gene of S. aureus in food sample.
Characterization and Genetic Variation of Vibrio cholerae Isolated from Clinical and Environmental Sources in Thailand
V. cholerae O1 El Tor variant and O139 were pre-dominating the pathogenic strains in Thailand, and Multidrug-resistant strains were observed and conferred resistance to ampicillin, azithromycin, nalidixic acid, sulfamethoxazole, tetracycline, and trimethoprim and harboured variants of the SXT elements.
Detection of Escherichia coli O157: H7 vt and rfb(O157) by multiplex polymerase chain reaction.
This modified multiplex PCR is a rapid, sensitive, and specific technique for detecting and differentiating E. coli O157: H7 and has the potential to be used as an alternative to conventional methods for the screening of O157; H7 strains isolated from raw meat.
O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139
A novel, sensitive locus-specific touchdown-multiplex polymerase chain reaction (TMPCR), which is based on two-stage amplification pertaining to multiplex PCR and conditional touchdown strategy, was
Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant Vibrio cholerae
The spread of multidrug-resistant (MDR) Vibrio cholerae necessitates the development of novel prevention and treatment strategies. This study aims to evaluate the in vitro antibacterial activity of
Dur-ing outbreaks of food poisoning , a number of DETECTION OF ESCHERICHIA COLI O 157 : H 7 VT AND RFB O 157 BY MULTIPLEX POLYMERASE CHAIN REACTION
A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for
Potential Antimicrobial Properties of Coffee Beans and Coffee By-Products Against Drug-Resistant Vibrio cholerae
Vibrio cholerae is the causative organism of the cholera epidemic, and it remains a serious global health problem, particularly the multidrug-resistant strain, despite the development of several