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Biphenyl-utilizing bacteria and their functional genes in a pine root zone contaminated with polychlorinated biphenyls (PCBs)
The findings improve understanding of BP degradation and carbon flow in soil, reveal the extent of culture bias, and may benefit bioremediation research by facilitating the development of molecular tools to detect, quantify and monitor populations involved in degradative processes.
Identification of Bacteria Utilizing Biphenyl, Benzoate, and Naphthalene in Long-Term Contaminated Soil
The results of this study show that many biphenyl/benzoate-assimilating bacteria derive carbon also from naphthalene, pointing out broader biodegradation abilities of some soil microbiota.
Pseudomonads Rule Degradation of Polyaromatic Hydrocarbons in Aerated Sediment
- J. Wald, Miluše Hroudová, J. Jansa, B. Vrchotová, T. Macek, Ondrej Uhlik
- BiologyFront. Microbiol.
- 19 November 2015
The results indicated an apparent dominance of pseudomonads in the sequestration of carbon from naphthalene and potential degradation of other PAHs upon aeration of the sediment at both 20 and 10°C.
Whole-Cell MALDI-TOF MS Versus 16S rRNA Gene Analysis for Identification and Dereplication of Recurrent Bacterial Isolates
It is indicated that a similarity-based MALDI-TOF MS approach can be routinely used for efficient dereplication of isolates for downstream analyses, with minimal loss of unique organisms.
Phyto/rhizoremediation studies using long-term PCB-contaminated soil
- M. Mackova, P. Prouzová, T. Macek
- Environmental ScienceEnvironmental science and pollution research…
- 13 October 2009
10 years of studies using long-term PCB-contaminated soil from a dumpsite in South Bohemia, targeted for the use of plants and their cooperation with microorganisms in the root zone, show how the efficiency of PCB removal is dependent on the plant used.
Effects of Secondary Plant Metabolites on Microbial Populations: Changes in Community Structure and Metabolic Activity in Contaminated Environments
- L. Musilová, Jakub Ridl, M. Polívková, T. Macek, Ondrej Uhlik
- Environmental ScienceInternational journal of molecular sciences
- 29 July 2016
The presented review supports the “secondary compound hypothesis” and demonstrates the potential of SPMEs for increasing the effectiveness of bioremediation processes.
DNA-based stable isotope probing: a link between community structure and function.
Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish…
This study shows the possibility of using MALDI-TOF MS and MalDI Biotyper for the fast and relatively nonlaborious identification/classification of soil isolates and demonstrates the dominant role of employing 16S rRNA gene analyses for the identification of recently isolated strains that can later fill the gaps in the protein-based identification databases.
Bacterial Degradation of Polychlorinated Biphenyls
Most microbe bioremediation processes take advantage of indigenous microorganisms, although some rely on the introduction of bacterial or fungal strains.
Phytoextraction of Heavy Metals: A Promising Tool for Clean-Up of Polluted Environment?
- Jachym Suman, Ondrej Uhlik, J. Viktorová, T. Macek
- Environmental ScienceFront. Plant Sci.
- 16 October 2018
This review focused on three basic HM phytoextraction strategies that differ in the type of plant species being employed: natural hyperaccumulators, fast-growing plant species with high-biomass production and, potentially, plants genetically engineered toward a phenotype that favors efficient HM uptake and boosted HM tolerance.