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The possibility that some or all of the viral proteins, gp70, p30, and the histocompatibility antigen, H-2, function as the tumor-specific transplantation antigen (TSTA) of the R-MuLV-induced leukemia, RBL-5, and also in the secondary in vitro induction of cytotoxic T lymphocytes (CTL), was investigated. The antigen was obtained by isolating the plasma(More)
Secondary induction of in vitro cytotoxic T lymphocytes in a syngeneic system has been achieved with plasma membrane, both in the particulate and solubilized forms. Both the induction and the lytic phases were shown to be immunologically specific. The effector cells generated were completely susceptible to treatment with anti-theta antibody and complement,(More)
When lymphocytes obtained from W/Fu rats primed with BCG are cultured in the presence of PPD, they elaborate a factor that is capable of potentiating the specific in vitro generation of cytotoxic lymphocytes to syngeneic (C58NT)D lymphoma cells and to BN alloantigen. Purification of the factor, achieved by gel filtration on Sephadex G-100, was facilitated(More)
The parameters of the secondary in vitro generation of syngeneic CTL by solubilized tumor antigens have been investigated. With PM and SPM, stimulation was achieved over a 10-fold antigen concentration, 5 microgram/ml to 0.5 microgram/ml, and with whole cells the optimum R/S cell ratio was 80:1 to 100:1. Assuming a 50-fold enrichment of plasma membrane(More)
Plasma membranes purified from RBL-5 leukemia cells and solubilized with Na deoxycholate were fractionated on an Ultrogel AcA34 column. Fractions containing most of the tumor-rejection activity but low amounts of gp70 and p30 were consolidated and used to hyperimmunize semisyngeneic, CBF1, hybrid mice. Using the complement-dependent cytotoxicity assay, we(More)