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We experimentally demonstrate the high-sensitivity optical monitoring of a micromechanical resonator and its cooling by active control. Coating a low-loss mirror upon the resonator, we have built an optomechanical sensor based on a very high-finesse cavity (30 000). We have measured the thermal noise of the resonator with a quantum-limited sensitivity at(More)
The aim of this paper is to propose a complete normalized study of a diaphragm's behavior under classical excitation encountered in microsystem: pneumatic, electrostatic, piezoelectric and magnetic. The principle of modeling is widely described in order to be easily applied to other structure. First, a traditional analytical solution is recalled for the(More)
The electric field is commonly used in microdevices to handle, treat, or monitor living cells for various biological or biomedical applications (cells electrofusion, gene electrotransfer, drugs injection, cell sorting, …). Dielectrophoresis (DEP) forces, using stationary waves (conventional DEP) or traveling waves, are widely used for the cell handling or(More)
This paper describes the fabrication and use of a biomimetic microfluidic device for the monitoring of a functional porin reconstituted within a miniaturized suspended artificial bilayer lipid membrane (BLM). Such a microfluidic device allows for (1) fluidic and electrical access to both sides of the BLM and (2) reproducible membrane protein insertion and(More)
This paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on the plasma membrane and on the intra cellular components occur, modifying the behavioral response of cells exposed to drugs or(More)
Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium(More)
A simple and versatile methodology has been developed for the simultaneous measurement of multiple concentration profiles of colourants in transparent microfluidic systems, using a conventional transmitted light microscope, a digital colour (RGB) camera and numerical image processing combined with multicomponent analysis. Rigorous application of the(More)
In this paper, we present a novel microfluidic system dedicated to the application of ultra short pulses (i.e. nanopulses) on cells and the visualization of their effects. Cell plasma membranes can be rendered permeable by the use of nanosecond pulsed electric field. In conventional macroscopic electroporation chambers, the typical pulse duration is on the(More)
The biological mechanisms induced by the application of nanosecond pulsed electric fields (nsPEFs: high electrical field amplitude during very short duration) on cells remain partly misunderstood. In this context, there is an increasing need for tools that allow the delivering of such pulses with the possibility to monitor their effects in real-time. Thanks(More)