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The aim of this paper is to propose a complete normalized study of a diaphragm's behavior under classical excitation encountered in microsystem: pneumatic, electrostatic, piezoelectric and magnetic. The principle of modeling is widely described in order to be easily applied to other structure. First, a traditional analytical solution is recalled for the(More)
This paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on the plasma membrane and on the intra cellular components occur, modifying the behavioral response of cells exposed to drugs or(More)
This paper describes the fabrication and use of a biomimetic microfluidic device for the monitoring of a functional porin reconstituted within a miniaturized suspended artificial bilayer lipid membrane (BLM). Such a microfluidic device allows for (1) fluidic and electrical access to both sides of the BLM and (2) reproducible membrane protein insertion and(More)
Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium(More)
In this paper, we present a novel microfluidic system dedicated to the application of ultra short pulses (i.e. nanopulses) on cells and the visualization of their effects. Cell plasma membranes can be rendered permeable by the use of nanosecond pulsed electric field. In conventional macroscopic electroporation chambers, the typical pulse duration is on the(More)
In MEMS, Electromagnetic field is a way to manipulate droplets, beads or cells without direct contact. In particular, dielectrophoresis is often used in BioMEMS applications, for cell handling operations such as moving, sorting, trapping or deforming cells. In this paper, a 3D focusing geometry for the electrical field is proposed, that permit the trapping(More)
Due to scaling laws, in microfluidic, flows are laminar. Consequently, mixing between two liquids is mainly obtained by natural diffusion which may take a long time or equivalently requires centimetre length channels. To reduce time and length for mixing, it is possible to generate chaotic-like flows either by modifying the channel geometry or by creating(More)
This paper proposes to monitor the spheroid’s permeabilization within a dedicated microfluidic device using electrorotation analyses. The combination of two electric solicitations, the negative dielectrophoresis force (nDEP) for the spheroid trapping and the electrorotation torque for its dielectric characterization, is used. An estimation of the spheroid(More)
The use of microfluidic devices for the handling and analysis of suspensions of colloidal gold particles is presented. The plasmonic particles are detected via their resonant light scattering (RLS) in a simple and versatile LED-based dark field illumination geometry. RLS enables both microscopic imaging and microspectroscopy. The measurement of the(More)