Olivia du Roure

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We measure dynamic traction forces exerted by epithelial cells on a substrate. The force sensor is a high-density array of elastomeric microfabricated pillars that support the cells. Traction forces induced by cell migration are deduced from the measurement of the bending of these pillars and are correlated with actin localization by fluorescence(More)
Fluorescence videomicroscopy and scanning force microscopy were used to follow, in real time, chromatin assembly on individual DNA molecules immersed in cell-free systems competent for physiological chromatin assembly. Within a few seconds, molecules are already compacted into a form exhibiting strong similarities to native chromatin fibers. In these(More)
The polymerization of actin in filaments generates forces that play a pivotal role in many cellular processes. We introduce a novel technique to determine the force-velocity relation when a few independent anchored filaments grow between magnetic colloidal particles. When a magnetic field is applied, the colloidal particles assemble into chains under(More)
Whereas the adhesion and migration of individual cells have been well described in terms of physical forces, the mechanics of multicellular assemblies is still poorly understood. Here, we study the behavior of epithelial cells cultured on microfabricated substrates designed to measure cell-to-substrate interactions. These substrates are covered by a dense(More)
Fibrosis is a hallmark of human white adipose tissue (WAT) during obesity-induced chronic inflammation. The functional impact of increased interstitial fibrosis (peri-adipocyte fibrosis) on adjacent adipocytes remains unknown. Here we developed a novel in vitro 3D culture system in which human adipocytes and decellularized material of adipose tissue (dMAT)(More)
Actin filaments play a fundamental role in cell mechanics: assembled into networks by a large number of partners, they ensure cell integrity, deformability, and migration. Here we focus on the mechanics of the dense branched network found at the leading edge of a crawling cell. We develop a new technique based on the dipolar attraction between magnetic(More)
We report measurements of the adhesion forces between single E-cadherin fragments anchored on solid surfaces. These fragments consist of the two outermost extracellular domains of the protein. The specificity of the measured rupture forces was demonstrated by Ca2+ exchange experiments. Two series of experiments were performed using two linkers of different(More)
We combine technical, experimental, and theoretical efforts to investigate the collective dynamics of artificial microcilia in a viscous fluid. We take advantage of soft lithography and colloidal self-assembly to devise microcarpets made of hundreds of slender magnetic rods. This novel experimental setup is used to investigate the dynamics of extended cilia(More)
We investigate experimentally and theoretically the dynamics of paramagnetic microrods anchored to a surface and driven by a precessing magnetic field. We identify two distinct regimes, corresponding to extended domains in the (ω,θ(B)) plane, where ω and θ(B) are, respectively, the frequency and inclination of the driving field. At low frequencies, the(More)
Gels are a functional template for micro-particle fabrication and microbiology experiments. The control and knowledge of their mechanical properties is critical in a number of applications, but no simple in situ method exists to determine these properties. We propose a novel microfluidic based method that directly measures the mechanical properties of the(More)