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We explored the feasibility of a strategy based on transposons to generate identified mutants of most Caenorhabditis elegans genes. A total of 1088 random new insertions of C. elegans transposons Tc1, Tc3, and Tc5 were identified by anchored PCR, some of which result in a mutant phenotype.
The Yule-Nielsen curve-fitting parameter n is derived in terms of the point spread function describing the scattering of light in paper substrates upon which are printed halftone dot or line patterns. The value of n is shown not to depend strongly on either screen frequency or area coverage at low area coverages or highlight regions. However, when the(More)
BACKGROUND Late diabetic complications cannot be prevented totally by current antidiabetic strategies. Therefore, new therapeutic concepts of insulin replacement such as pancreas transplantation are evolving. Due to the shortage of human donor organs, transplantation of microencapsulated xenogeneic pancreatic islet cells has attracted considerable(More)
One hundred fifty million people suffer from diabetes mellitus worldwide. Modern exogenous insulin therapy cannot prevent late complications. Islet cell transplantation could be a sufficient therapeutic option but the shortage of human organs limits this option. The use of xenogeneic porcine islet cells may also be a viable alternative. One way to manage(More)
The ability to implant genetically modified cells in immuno-isolating materials has much potential for therapeutic use. This review presents the different types of polymer that can be used for encapsulation of cells and the recent use of genetically modified, encapsulated and thus immunoprotected cells for the treatment of diseases in animal models and(More)
INTRODUCTION Transplanted cells, especially islet cells, are likely to become apoptotic due to local hypoxia leading to graft dysfunction. Isolated pancreatic islet cells depend on the diffusion of oxygen from the surrounding tissue; therefore, access to sufficient oxygen supply is beneficial, particularly when microcapsules are used for immunoisolation in(More)
INTRODUCTION Diabetes mellitus may be treated with pancreatic islet cell transplantation. The use of xenogenic islet cells may overcome the shortage of human donor organs. Microencapsulation seems to be a promising method for immunoprotection. Since isolation, purification, encapsulation, and transplantation of islet cells are labor-intensive,(More)
Using a standardised psychological questionnaire (self-estimation scale, personality-inventory FPI of Fahrenberg and Selg) and serial examinations, the effectiveness of a therapeutic concept combining drug, environmental and group therapy was tested. The psychological parameters objectified stabilisation of the psychological-autonomic and emotional areas as(More)
A measurement technique is presented that permits the characterization of the regularity of low-coverage halftone patterns common to many reproduction processes. The technique is based on a scanning microdensitometer, which examines individual halftone cells and compares them with their neighbors. It is believed, but not shown, that the number descriptor(More)
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