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Pathways of synthesis of the α-reactive carbonyl compound methylglyoxal (MG) in prokaryotes are described in this review. Accumulation of MG leads to development of carbonyl stress. Some pathways of MG formation are similar for both pro- and eukaryotes, but there are reactions specific for prokaryotes, e.g. the methylglyoxal synthase reaction. This reaction(More)
We described a spectrophotometric method for measuring hemoglobin peroxidase activity in human plasma using o-dianisidine (o-DA) as the substrate and myeloperoxidase specific inhibitor 4-aminobensoic acid hydrazide (ruling out the probable contribution of myeloperoxidase to the measured parameter value). The optimal conditions (pH 5.5; 2 mM H2O2) have been(More)
BACKGROUND Nitric oxide (NO) and its metabolites can nitrosylate hemoglobin (Hb) through the heme iron. Nitrihemoglobin (nitriHb) can be formed as result of porphyrin vinyl group modification with nitrite. However, in those with diabetes the non-enzymatic glycation of Hb amino acids residues (the Maillard reaction) can take place. The objectives of this(More)
Prooxidant and antioxidant properties of nitric oxide (NO) during oxidative stress are mostly dependent on its interaction with reactive oxygen species, Fe ions, and hemoproteins. One form of NO storage and transportation in cells and tissues is dinitrosyl iron complexes (DNIC), which can bind with both low-molecular-weight thiols and proteins, including(More)
The Maillard reaction is the key process in protein modification during pathologies connected with carbonyl stress. It was shown in system modeling that Maillard reaction interaction of L-lysine (L-lys) with methylglyoxal (MG) led to the formation of compounds reducing methemoglobin (metHb). Under the above conditions and in the presence of(More)
Dinitrosyl iron complexes (DNICs) are physiological NO derivatives and account for many NO functions in biology. Polyfunctional dipeptide carnosine (beta-alanyl-l-histidine) is considered to be a very promising pharmacological agent. It was shown that in the system containing carnosine, iron ions and Angeli’s salt, a new type of DNICs bound with carnosine(More)
Methylglyoxal (MG) is one of the physiological glucose metabolites formed in living organisms. The data on the influence of MG on different internal systems of eukaryotic cells, including the central signaling pathways, are been discussed in the review. The central signaling pathways are stress-activated and sensitive to the action of reactive oxygen(More)
Destructive effect of superoxide anions O2- derived from KO(2) or xanthine-xanthine oxidase system on dinitrosyl-iron complexes bound with bovine albumin or methemoglobin (DNIC-BSA or DNIC-MetHb) was demonstrated. The sensitivity of DNIC-BSA synthesized by the addition of DNIC with cysteine, thiosulfate or phosphate (DNIC-BSA-1, DNIC-BSA-2 or DNIC-BSA-3,(More)
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