Olga N Veshkurova

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From seeds of Nigella sativa L. (Ranunculaceae), an endemic plant of Uzbekistan, two novel defensins named Ns-D1 and Ns-D2, were isolated and sequenced. The peptides differ by a single amino acid residue and show high sequence similarity to Raphanus sativus L. defensins Rs-AFP1 and Rs-AFP2. The Ns-D1 and Ns-D2 defensins display strong although divergent(More)
The isolation and structure of a phytoalexin, malvone A (2-methyl-3-methoxy-5,6-dihydroxy-1,4-naphthoquinone) is reported. Malvone A formation is induced in Malva sylvestris L. by the plant pathogen Verticillium dahliae. In a turbimetric assay for toxicity to V. dahliae, it had an ED50 value of 24 microg/ml. The structure of malvone A was determined by MS(More)
A novel lipid-transporting protein (Ns-LTP1) has been isolated from seeds of the garden fennel flower Nigella sativa. The molecular mass, N-terminal amino acid sequence, and amino acid composition of the protein have been determined. Ns-LTP1 has a molecular mass of 9602 Da and contains eight cysteine residues which form four disulfide bridges. The protein(More)
We have previously studied the chemical composition of essential oil (EO) from seeds of Anethum graveolens from Xinjiang Autonomous District in the PRC [1]. The component composition of essential oils is known to depend on the habitat. It seemed interesting to compare the composition and biological activity of EO from dill seeds growing in China and(More)
A non-specific lipid-transfer peptide (nsLTP) with fungicidal activity was isolated from Daucus carota sativa carrot seeds. Peptides were purified by a method including aqueous extraction, anion-exchange chromatography over CM-TSK-650M, and HPLC over a column of 250/8/4 Protein@Peptide C18 using an acetonitrile gradient. The molecular weight of the peptide(More)
Four peptides of molecular weights 1.148, 4.68, 5.41, and 9.086 kDa with antioxidant activity were isolated from chickpea sprout using two-step ion-exchange chromatographic and HPLC techniques for the first time. The partial N-terminal amino acid sequences of the peptide named YZDBCM-1 (9.086 kDa) was determined as(More)
Three-day etiolated seedlings of the cotton plant Gossypium hirsutum, variety 48-80 in an amount of i0 g were homogenized in buffer A (50 mM Tris-HCl, 5 mM EDTA, 2.5 mM MgCI=, 50 mM KCI, pH 7.4). Homogenization and all the following operations were performed strictly at 2-4°C. Large cell organelles (nuclei, chloroplasts, and mitochondria) were eliminated by(More)
Phytophthorosis is one of the most economically significant potato diseases in the world and can led to total loss of a harvest. At present, the only effective means for limiting the spread of this disease in agricultural mass production is multiple applications of fungicides, residues of which persist in the final product. Antifungal properties, in(More)
The biosynthesis and spectrum of PR-proteins isolated from leaves of eight cotton varieties that were affected by Verticillium dahliae were studied. It was shown that 1,3-β-glucanase, chitinase, and catalase activity increased in response to the pathogen. 1,3-β-Glucanase, three of its isoforms, and chitinase were isolated.