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OBJECTIVES The objective of this study was to determine the effect of LLDL therapy on the gene expression of osteoblast markers of growth and differentiation. MATERIALS AND METHODS The MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1-1.5 W and 3-4 J, and gene expressions (Runx-2, alkaline phosphatase [ALP], type I collagen [Col-I],(More)
Human osteoblasts isolated from bone tissue samples have their own specific antigen profile but also share expression of antigens that are characteristic of other immunocompetent cells. Given that these findings come from studies performed in primary cultures of human osteoblasts, it was decided to test whether these antigen profiles and functional(More)
OBJECTIVES The aim of this cross-sectional study was to evaluate the periodontal status and oral microbiological patterns of a population with end-stage renal disease (ESRD), undergoing haemodialysis (HD). DESIGN This was a cross-sectional study, involving 52 patients from the Nephrology Department and 52 matched control subjects. MATERIALS AND METHODS(More)
Laser is a simple, noninvasive technique that has proven useful for treating damaged tissue. However, its effects on bone regeneration and the mechanisms involved are poorly understood. The objective of this study was to evaluate the effects on MG-63 cell proliferation of application of a pulsed diode laser (Ezlase) of 940 nm at low energy levels. After 24(More)
BACKGROUND Pressure ulcers (PUs) are prevalent and chronic wounds that require significant time to heal and the search for new treatments to reduce healing time is ongoing. We describe our experience with platelet-rich plasma to facilitate PU healing. CASE An 86-year-old woman residing in a long-term care facility developed a grade III PU on her right(More)
Previous in vivo and in vitro studies have reported that low-level diode laser therapy induces a biostimulatory effect, such as cell proliferation. The aim of the present study was to evaluate whether the laser irradiation of osteoblast-like cells (MG-63) can modify alkaline phosphatase activity (ALP), antigenic profile, and phagocytic capacity. The MG-63(More)
The antigenic profile of human osteoblasts was previously analyzed by our group using primary cultures as study samples. These studies suggested a novel functional approach to this cell population. Osteoblasts have a characteristic antigenic profile and share antigens in common with other cell populations that also originate in the bone marrow. Some of the(More)
OBJECTIVES The aims of our study were to verify the presence of viable osteoblasts in samples of bone tissue obtained by drilling or from cortico-cancellous bone blocks and to assess their growth and differentiation capacities. MATERIALS AND METHODS Bone tissue samples were processed independently and cultured in Dulbecco's modified Eagle medium, in a CO2(More)
Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used in bone tissue repair treatment for their pharmacological action. The objective of this study was to determine the effect of aspirin, on osteoblast growth, using MG63 cell line as osteoblast model. MTT spectrophotometry results showed that 20, 100, and 1000 μM aspirin doses have an inhibitory(More)
The antigenic phenotype of cultured human osteoblast-like cells, their ability to phagocytose particles of different nature and size, and their capacity to stimulate allogeneic T cells suggest that they are related to other cell populations with which they may also have immunological functions in common. The objective of this study was to investigate the(More)