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Major histocompatibility complex (MHC) class II molecules are membrane-anchored heterodimers that present antigenic peptides to T cells. Expression of these molecules in soluble form has met limited success, presumably due to their large size, heterodimeric structure and the presence of multiple disulfide bonds. Here we have used directed evolution and(More)
Identification of CD4+ T-cell epitopes is a critical step in studying and modulating the immune responses to tumors, infectious agents, and autoantigens. Here we report a facile, accurate, and high-throughput method for CD4+ T-cell epitope identification using yeast displaying pathogen-derived peptide library. A library of DNA fragments that encode all the(More)
1. Introduction Variation coupled to selection is the hallmark of natural evolution. Although there is no full agreement concerning the best way to create variation , mutation or recombination (1), computational simulation studies have demonstrated the importance of homologous recombination in the evolution of biological systems (2,3). As compared to random(More)
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