Oleg V Batishchev

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BACKGROUND H5N1 influenza vaccines, including live intranasal, appear to be relatively less immunogenic compared to seasonal analogs. The main influenza virus surface glycoprotein hemagglutinin (HA) of highly pathogenic avian influenza viruses (HPAIV) was shown to be more susceptible to acidic pH treatment than that of human or low pathogenic avian(More)
Influenza A virus matrix protein M1 is one of the most important and abundant proteins in the virus particles broadly involved in essential processes of the viral life cycle. The absence of high-resolution data on the full-length M1 makes the structural investigation of the intact protein particularly important. We employed synchrotron small-angle X-ray(More)
The process of electroporation of bilayer lipid membranes made of diphytanoylphosphatidylcholine (DPhPC) has been studied. DPhPC combines a polar part typical for lipids of eukaryotic cells and branched isoprenoid chains forming the hydrophobic “tails” of archaea lipids. From the experimental dependence of the average lifetime on the magnitude of the(More)
Formation of bilayer lipid membrane (BLM) by Montal-Mueller technique across a small aperture in a partition film traditionally requires coating of the aperture with a hydrophobic substance, often just an organic solvent. However, we demonstrate here that the most effective coating is not strictly hydrophobic but rather provides water/oil repellent(More)
UNLABELLED Influenza virus is taken up from a pH-neutral extracellular milieu into an endosome, whose contents then acidify, causing changes in the viral matrix protein (M1) that coats the inner monolayer of the viral lipid envelope. At a pH of ~6, M1 interacts with the viral ribonucleoprotein (RNP) in a putative priming stage; at this stage, the(More)
Theoretical model of structure of through pore in lipid bilayer is developed. We assumed that hydrophobic void can appear between monolayers of the membrane. The void is supposed to be filled by organic solvent or low-polarity impurities, which are usually present in artificial and cell membranes. The pore edge was divided to three regions: two monolayer(More)
Proton diffusion along biological membranes is vitally important for cellular energetics. Here we extended previous time-resolved fluorescence measurements to study the time and temperature dependence of surface proton transport. We determined the Gibbs activation energy barrier ΔG ‡r that opposes proton surface-to-bulk release from Arrhenius plots of (i)(More)
Liquid-ordered lipid domains, also called rafts, are assumed to be important players in different cellular processes, mainly signal transduction and membrane trafficking. They are thicker than the disordered part of the membrane and are thought to form to compensate for the hydrophobic mismatch between transmembrane proteins and the lipid environment.(More)
The heating and evolution of ionized single cluster in a strong laser field is studied with molecular dynamics simulation. The electron temperature increase and particle expelling from evolution of deuterium and iron clusters are discussed. It is shown that the optimal regime for effective electron heating corresponds to laser field comparable to the(More)