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Cell biology, as monitored with the fluorescent indicator dyes Alamar Blue and 5-carboxyfluorescein diacetate acetoxymethyl ester (CFDA-AM), and lens optical quality, as measured with an in vitro scanning laser system, have been used to evaluate in vitro the condition of porcine lenses after being placed in a culture medium. The measurements, beginning from(More)
PURPOSE To establish the in vitro action spectrum for acute UV cataractogenesis using whole cultured lenses. The recovery pattern of the induced cataract was also investigated. METHODS Aseptically dissected porcine lenses were cultured in glass chambers. At 1 week, lenses were exposed to a predetermined UV energy (J/cm(2)) at specific wavebands ranging(More)
The main objective of this study was to investigate the effect of in vivo hyperbaric oxygen (HBO) treatment of albino guinea pigs on ocular refractive state and optical properties of the lens in vitro, as well as on the integrity of the mitochondria of the lens. The animals were treated 30-35 times (2.5-3 months) or 70 times (6 months) with HBO. An(More)
PURPOSE The use of the alamarBlue fluorescence dye for cellular study of UV-induced photodamage in cultured ocular lenses was examined by comparing the results from the fluorometric assay to lens optical quality using a scanning laser system to measure the focal lengths of the lenses following UVB treatment. METHODS Excised porcine lenses were cultured in(More)
PURPOSE The aim of this study was to determine the impact of diabetes in patients with and without dry eyes (DE) on central corneal thickness (CCT) measurements. METHODS Eighty-six subjects (51 diabetics and 35 controls) participated in the study. Ultrasound pachymetry was used to measure the CCT, whereas tear break-up time (TBUT) and Schirmer tests were(More)
The phototoxicity of ultraviolet A (UVA) alone and UVA plus ultraviolet B (UVB) combined on cultured porcine lenses was investigated by analyzing cellular function as measured with a fluorescence bioassay approach and optical integrity, in terms of sharpness of the lens focus as measured with a scanning laser system. The bioassay consisted of(More)
PURPOSE To evaluate in vitro methods for testing the toxicity of the surfactants, sodium dodecyl sulfate (SDS) and benzalkonium chloride (BAK), and Ultraviolet (UV)-B radiation to the bovine lens. METHODS Lenses were dissected from bovine eyes--obtained from a local abattoir--and incubated in M199 culture medium at 37 degrees C, with 4% CO(2) and 96% air(More)
The occupational radiation exposure levels and the ocular health status of a sample of glassblowers in southern Ontario were evaluated. The form of ocular protection was also assessed. Measurements of radiant emissions from different molten glass materials were carried out. A total of six glassblowing installations, 15 glassblowers and 42 non-glassblowers(More)
The aims of this study were to investigate the levels of optical radiation exposure in glassblowing and to determine type(s) of protective eyewear commonly used. Radiometric measurements of radiant emissions from different molten glass materials and heating systems were carried out in six installations. Spectral transmittance curves of available protective(More)
OBJECTIVE To investigate if the levels of optical radiation hazards in glassblowing are well classified according to the hazard types defined in the Canadian Standards Association (CSA) standard for industrial eye protectors. METHODS We carried out radiometric measurements, and questionnaire survey in 4 university glassblowing laboratories, and 3 private(More)