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Journals and Conferences
Fluorescent base analogues in DNA are versatile probes of nucleic acid-nucleic acid and nucleic acid-protein interactions. New peptide nucleic acid (PNA) based probes are described in which the intercalator dye thiazole orange (TO) serves as a base surrogate. The investigation of six TO derivatives revealed that the linker length and the conjugation site… (More)
Thiazole orange is shown to possess characteristics of a universal base while maintaining duplex stability. Its fluorescence properties allowed distinction between matched and single mismatched hybridisation.
The site-selective conjugation of peptide nucleic acids (PNA) with fluorescent reporter groups is essential for the construction of hybridisation probes that can report the presence of a particular DNA sequence. This paper describes convergent methods for the solution- and solid-phase synthesis of multiply labelled PNA oligomers. The solid-phase synthesis… (More)
The fluorescence of thiazole orange as artificial base in PNA was investigated in a nearest neighbour analysis; library-to-library hybridisation allowed the identification of probe sequences suitable for homogeneous DNA detection.