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High-Activity P-Glycoprotein, Multidrug Resistance Protein 2, and Breast Cancer Resistance Protein Membrane Vesicles Prepared from Transiently Transfected Human Embryonic Kidney 293-Epstein-Barr…
- J. Karlsson, C. Heddle, +4 authors T. Andersson
- Biology, Medicine
- Drug Metabolism and Disposition
- 1 April 2010
The production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney 293-Epstein-Barr virus nuclear antigen cells transiently transfected using a Gateway-adapted pCEP4 plasmid are described. Expand
Development of a generic transient transfection process at 100 L scale
A generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfections reagent for the production of recombinant IgG is developed, showing to be reproducible at 10, 50, and 100-L scale expressing between 5 and 8 mg L−1 IgG. Expand
The SARS-CoV-2 N Protein Is a Good Component in a Vaccine
Dutta and co-workers suggest in a recent letter (1) that the SARS-CoV-2 nucleoprotein (N) might be a good vaccine target.….