Oksan Volkova

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Pseudomonas’ IncP-7 plasmids play significant role in the environmental biodegradative potential and sometimes carry antibiotic-resistance genes. Rms148 plasmid was used as archetypal P-7 plasmid in microbiological incompatibility studies for more then 30 years. However, the structure of its basic replicon was not described until now; furthermore, the(More)
Both caprolactams and salicylate biodegradation by Pseudomonas salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (scpA) has been detected in SAL/CAP plasmids and partially sequenced. The scpA gene was(More)
A basic replicon of the naphthalene degradation plasmid pFME5 (80 kb, IncP-7) has been constructed and sequenced. The nucleotide sequence of pFME5mini is almost identical to replicons of the pND6-1 subgroup, which was separated based on the repA-oriV homology in our previous work. The basic replicon of pFME5 is capable of replication and stable maintenance(More)
Nuclear factor kappa B (NFkappaB) is a ubiquitous nuclear transcription factor that regulates the expression of a number of genes involved in cell survival, immune and inflammatory processes. It has been hypothesized that after nerve injury, the release of specific cytokines may provide a stimulus for activation of the transcription factor NF-kappaB in(More)
726 Investigation of molecular mechanisms of the emergence and outspread of the capacity of microorr ganisms for oxidation of xenobiotic compounds that had not previously existed in nature is not only imporr tant for the science of evolutionary biology, but is also of practical significance for ecology, biotechnology, and metabolic engineering. Caprolactam(More)
The structural diversity of basic replicons and repB gene was analyzed for the first time in a large collection of IncP-7 plasmids by PCR, restriction endonuclease analysis, and partial sequencing. It was found that the DNA fragment that contains the gene for UvrD-like helicase RepB is a part of all known P-7 replicons, but often acts as a hot insertion(More)
A mini-replicon was obtained for the caprolactam/salicylate degradation plasmid pBS270 (105 kb) of incompatibility group P-7 from Pseudomonas bacteria, and its nucleotide sequence was determined. A new gene that encodes a TrfA-like replication initiator was found on this replicon. The level of homology between this replication initiator and known proteins(More)
At least three vasopressinergic brain systems are involved in inflammatory reaction. The first one is VP neurons of the bed nucleus of stria terminalis, projecting to antipyretic center in the preoptic forebrain region. The second system is neurons of the parvocellular subnucleus of the hypothalmic paraventricular nucleus. VP from these neurons reaches(More)